Anti-Phospho-YAP1 (S127) Antibody (RACO0087)
- SKU:
- RACO0087
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | Phospho-YAP1 (S127) Antibody |
| Product SKU: | RACO0087 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Phospho-YAP1 (S127) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750). |
| シノニム: | Transcriptional coactivator YAP1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1, YAP65 |
 | IHC image of RACO0087 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in HepG2 whole cell lysate treated with Calyculin A or not). All lanes Phospho-YAP1 antibody at 0. (3µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 65 KDa. Observed band size: 65 KDa. |
| UniProt Code: | P46937 |
| NCBI GenInfo Identifier: | 294862479 |
| NCBI Gene ID: | 10413 |
| NCBI Accession: | P46937.2 |
| UniProt Secondary Accession: | P46937,Q7Z574, B4DTY1, B7ZA01, E3WEB5, E3WEB6, E9PRV2 F5H202, K0KQ18, K0KYZ8, K0L195, K0L1G3, |
| UniProt Related Accession: | P46937 |
| Molecular Weight: | 504 |
| NCBI Full Name: | Transcriptional coactivator YAP1 |
| NCBI Synonym Full Names: | Yes-associated protein 1 |
| NCBI Official Symbol: | YAP1 |
| NCBI Official Synonym Symbols: | YAP; YKI; COB1; YAP2; YAP65 |
| NCBI Protein Information: | transcriptional coactivator YAP1; yorkie homolog; protein yorkie homolog; yes-associated protein 2; 65 kDa Yes-associated protein; yes-associated protein YAP65 homolog |
| UniProt Protein Name: | Transcriptional coactivator YAP1 |
| UniProt Synonym Protein Names: | Protein yorkie homolog; Yes-associated protein YAP65 homolog |
| Protein Family: | Transcriptional coactivator |
| UniProt Gene Name: | YAP1 |
| UniProt Entry Name: | YAP1_HUMAN |