Anti-Phospho-RELA-S311 Antibody (CABP0445)
- SKU:
- CABP0445
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| 抗体名: | Anti-Phospho-RELA-S311 Antibody |
| 抗体コード: | CABP0445 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic phosphorylated peptide around S311 of human NF-kB p65 (NP_068810.3). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa |
| 免疫原: | A synthetic phosphorylated peptide around S311 of human NF-kB p65 (NP_068810.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FKSI M |
| 遺伝子ID: | 5970 |
| Uniprot: | Q04206 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 58kDa/59kDa/60kDa |
| 観察された分子量: | 65KDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-NF-kB p65-S311 antibody (CABP0445) at 1:500 dilution.HeLa cells were treated by TNF-a (20 ng/ml) at 37'C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-NF-kB p65-S311 antibody (CABP0445) at dilution of 1:100 (40x lens). |
| 同義語: | NFKB3, p65, NF-kB p65, RELA, CMCU |
| バックグラウンド: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex Molecular Function:actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway |
| NCBI Summary: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q04206 |
| NCBI GenInfo Identifier: | 62906901 |
| NCBI Gene ID: | 5970 |
| NCBI Accession: | Q04206.2 |
| UniProt Secondary Accession: | Q04206,Q6GTV1, Q6SLK1, |
| UniProt Related Accession: | Q04206 |
| Molecular Weight: | 59,910 Da |
| NCBI Full Name: | Transcription factor p65 |
| NCBI Synonym Full Names: | RELA proto-oncogene, NF-kB subunit |
| NCBI Official Symbol: | RELA |
| NCBI Official Synonym Symbols: | p65; NFKB3 |
| NCBI Protein Information: | transcription factor p65 |
| UniProt Protein Name: | Transcription factor p65 |
| UniProt Synonym Protein Names: | Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Protein Family: | Proline-rich P65 protein |
| UniProt Gene Name: | RELA |