Anti-Phospho-Histone H3.3 (T3) Antibody (RACO0001)
- SKU:
- RACO0001
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Application:
- ICC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | Phospho-Histone H3.3 (T3) Antibody |
| Product SKU: | RACO0001 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, ICC, FC |
| 推奨される希釈: | WB:1:500-1:5000, ICC:1:50-1:500 |
| 反応性: | Human, Mouse |
| 免疫原: | A synthesized peptide |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| シノニム: | Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B |
 | Immunocytochemistry analysis of RACO0001 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Overlay histogram showing Hela cells stained with RACO0001 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
 | Western Blot. Positive WB detected in Hela whole cell lysate 293 whole cell lysate NIH/3T3 whole cell lysate All lanes Phospho-Histone H3 (T3) antibody at 1. (1µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 16 KDa. Observed band size: 16 KDa. |
| UniProt Protein Function: | H3F3A: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42.12 Cellular Component: extracellular region; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:histone binding; nucleosomal DNA binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of cell growth; positive regulation of gene expression, epigenetic; RNA-mediated gene silencing; telomere organization and biogenesis |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Oct 2015] |
| UniProt Code: | P84243 |
| NCBI GenInfo Identifier: | 55977062 |
| NCBI Gene ID: | 3021 |
| NCBI Accession: | P84243.2 |
| UniProt Secondary Accession: | P84243,P06351, P33155, Q5VV55, Q5VV56, Q66I33, Q9V3W4 |
| UniProt Related Accession: | P84243 |
| Molecular Weight: | 15,328 Da |
| NCBI Full Name: | Histone H3.3 |
| NCBI Synonym Full Names: | H3 histone, family 3B |
| NCBI Official Symbol: | H3F3B |
| NCBI Official Synonym Symbols: | H3.3B |
| NCBI Protein Information: | histone H3.3 |
| UniProt Protein Name: | Histone H3.3 |
| UniProt Gene Name: | H3F3A |
| UniProt Entry Name: | H33_HUMAN |