Anti-Phospho-Histone H1.4 (T17) Antibody (RACO0014)
- SKU:
- RACO0014
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | Phospho-Histone H1.4 (T17) Antibody |
| Product SKU: | RACO0014 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | IHC:1:50-1:500, IF:1:30-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
| シノニム: | Histone H1.4, Histone H1b, Histone H1s-4, HIST1H1E, H1F4 |
 | IHC image of RACO0014 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of MCF-7 cells with RACO0014 at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | IHC image of RACO0014 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| UniProt Protein Function: | H1E: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nuclear heterochromatin; nucleus Molecular Function:chromatin DNA binding; protein binding |
| NCBI Summary: | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P10412 |
| NCBI GenInfo Identifier: | 121919 |
| NCBI Gene ID: | 3008 |
| NCBI Accession: | P10412.2 |
| UniProt Secondary Accession: | P10412,Q4VB25, |
| UniProt Related Accession: | P10412 |
| Molecular Weight: | 21,865 Da |
| NCBI Full Name: | Histone H1.4 |
| NCBI Synonym Full Names: | histone cluster 1, H1e |
| NCBI Official Symbol: | HIST1H1E |
| NCBI Official Synonym Symbols: | H1E; H1.4; H1F4; H1s-4; dJ221C16.5 |
| NCBI Protein Information: | histone H1.4 |
| UniProt Protein Name: | Histone H1.4 |
| UniProt Synonym Protein Names: | Histone H1b; Histone H1s-4 |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST1H1E |
| UniProt Entry Name: | H14_HUMAN |