Anti-Phospho-FOXO3 (S253) Antibody (RACO0065)
- SKU:
- RACO0065
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | Phospho-FOXO3 (S253) Antibody |
| Product SKU: | RACO0065 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Phospho-FOXO3 (S253) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. |
| シノニム: | Forkhead box protein O3, AF6q21 protein, FOXO3 |
 | IHC image of RACO0065 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of RACO0065 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in Hela whole cell lysate 293 whole cell lysate A549 whole cell lysate All lanes Phospho-FOXO3 antibody at 0.96µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 97 KDa. Observed band size: 97 KDa. |
| UniProt Protein Function: | FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. |
| UniProt Protein Details: | Protein type:Oncoprotein; DNA-binding; Transcription factor; Autophagy Chromosomal Location of Human Ortholog: 6q21 Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus Molecular Function:protein binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity; protein kinase binding Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; nerve growth factor receptor signaling pathway; initiation of primordial ovarian follicle growth; positive regulation of transcription, DNA-dependent; antral ovarian follicle growth; positive regulation of erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; ovulation from ovarian follicle; oocyte maturation; regulation of translation; regulation of transcription from RNA polymerase II promoter; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43524 |
| NCBI GenInfo Identifier: | 8134467 |
| NCBI Gene ID: | 2309 |
| NCBI Accession: | O43524.1 |
| UniProt Secondary Accession: | O43524,O15171, Q5T2I7, Q9BZ04, B4DVZ6, E1P5E6, |
| UniProt Related Accession: | O43524 |
| Molecular Weight: | |
| NCBI Full Name: | Forkhead box protein O3 |
| NCBI Synonym Full Names: | forkhead box O3 |
| NCBI Official Symbol: | FOXO3 |
| NCBI Official Synonym Symbols: | FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2 |
| NCBI Protein Information: | forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 |
| UniProt Protein Name: | Forkhead box protein O3 |
| UniProt Synonym Protein Names: | AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1 |
| Protein Family: | Forkhead box protein |
| UniProt Gene Name: | FOXO3 |
| UniProt Entry Name: | FOXO3_HUMAN |