Anti-Phospho-ERN1 (S724) Antibody (RACO0073)
- SKU:
- RACO0073
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | Phospho-ERN1 (S724) Antibody |
| Product SKU: | RACO0073 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IF |
| 推奨される希釈: | WB:1:500-1:5000, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Phospho-ERN1 (S724) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation (PubMed:9637683, PubMed:11175748, PubMed:12637535). The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis (PubMed:24508390). |
| シノニム: | Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, ERN1 |
 | Immunofluorescence staining of Hela cells with RACO0073 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Western Blot. Positive WB detected in 293 whole cell lysate A549 whole cell lysate Hela whole cell lysate (treated with Calyculin A or EGF). All lanes Phospho-ERN1 antibody at 0.75µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 110 kDa. Observed band size: 110 kDa. |
| NCBI Summary: | This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017] |
| UniProt Code: | O75460 |
| NCBI GenInfo Identifier: | 193806335 |
| NCBI Gene ID: | 2081 |
| NCBI Accession: | O75460.2 |
| UniProt Secondary Accession: | O75460,Q76MJ5, |
| UniProt Related Accession: | O75460 |
| Molecular Weight: | Observed: 110 kDaPredicted: 110 kDa |
| NCBI Full Name: | Serine/threonine-protein kinase/endoribonuclease IRE1 |
| NCBI Synonym Full Names: | endoplasmic reticulum to nucleus signaling 1 |
| NCBI Official Symbol: | ERN1 |
| NCBI Official Synonym Symbols: | IRE1; IRE1P; IRE1a; hIRE1p |
| NCBI Protein Information: | serine/threonine-protein kinase/endoribonuclease IRE1 |
| UniProt Protein Name: | Serine/threonine-protein kinase/endoribonuclease IRE1 |
| UniProt Synonym Protein Names: | Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a |
| Protein Family: | Serine/threonine-protein kinase/endoribonuclease |
| UniProt Gene Name: | ERN1 |
| UniProt Entry Name: | ERN1_HUMAN |