Anti-Phospho-EIF4E (S209) Antibody (RACO0052)
- SKU:
- RACO0052
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | Phospho-EIF4E (S209) Antibody |
| Product SKU: | RACO0052 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Phospho-EIF4E (S209) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. |
| シノニム: | Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F |
 | IHC image of RACO0052 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in 293 whole cell lysate HepG2 whole cell lysate treated with Calyculin A or not). All lanes Phospho-EIF4E antibody at 1(2µg)ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 25 KDa. Observed band size: 25 KDa. |
| UniProt Protein Function: | EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 4q23 Cellular Component: mRNA cap complex; stress granule; eukaryotic translation initiation factor 4F complex; cytoplasm; cytosol Molecular Function:protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding Biological Process: viral reproduction; translation; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; G1/S transition of mitotic cell cycle; lung development Disease: Autism, Susceptibility To, 19 |
| NCBI Summary: | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P06730 |
| NCBI GenInfo Identifier: | 1352435 |
| NCBI Gene ID: | 1977 |
| NCBI Accession: | P06730.2 |
| UniProt Secondary Accession: | P06730,Q96E95, B7Z6V1, D6RCQ6, |
| UniProt Related Accession: | P06730 |
| Molecular Weight: | 27,260 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 4E |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4E |
| NCBI Official Symbol: | EIF4E |
| NCBI Official Synonym Symbols: | CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1 |
| NCBI Protein Information: | eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein |
| UniProt Protein Name: | Eukaryotic translation initiation factor 4E |
| UniProt Synonym Protein Names: | eIF-4F 25 kDa subunit; mRNA cap-binding protein |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF4E |
| UniProt Entry Name: | IF4E_HUMAN |