Anti-PHLDA3 Antibody (CAB12666)
- SKU:
- CAB12666
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| 抗体名: | Anti-PHLDA3 Antibody |
| 抗体コード: | CAB12666 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human PHLDA3 (NP_036528.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | SKOV3, A-431 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human PHLDA3 (NP_036528.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTAA ATAT VLKE GVLE KRSG GLLQ LWKR KRCV LTER GLQL FEAK GTGG RPKE LSFA RIKA VECV ESTG RHIY FTLV TEGG GEID FRCP LEDP GWNA QITL GLVK FKNQ QAIQ TVRA RQSL GTGT LVS |
| 遺伝子ID: | 23612 |
| Uniprot: | Q9Y5J5 |
| セルラーロケーション: | Cytoplasm, Membrane, Peripheral membrane protein |
| 計算された分子量: | 13kDa |
| 観察された分子量: | 16kDa |
| 同義語: | PHLDA3, TIH1 |
| バックグラウンド: |
| UniProt Protein Function: | PHLDA3: p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. Belongs to the PHLDA3 family. Induced by p53/TP53; expression is directly activated by p53/TP53. p53/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 promoter. |
| UniProt Protein Details: | Protein type:Tumor suppressor Chromosomal Location of Human Ortholog: 1q31 Cellular Component: cytoplasm; plasma membrane Molecular Function:phosphatidylinositol 3-phosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-5-phosphate binding Biological Process: anatomical structure morphogenesis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of protein kinase B signaling cascade; positive regulation of apoptosis |
| UniProt Code: | Q9Y5J5 |
| NCBI GenInfo Identifier: | 74735291 |
| NCBI Gene ID: | 23612 |
| NCBI Accession: | Q9Y5J5.1 |
| UniProt Secondary Accession: | Q9Y5J5,Q53HD6, Q8NBW9, B2R5A4, |
| UniProt Related Accession: | Q9Y5J5 |
| Molecular Weight: | |
| NCBI Full Name: | Pleckstrin homology-like domain family A member 3 |
| NCBI Synonym Full Names: | pleckstrin homology like domain family A member 3 |
| NCBI Official Symbol: | PHLDA3 |
| NCBI Official Synonym Symbols: | TIH1 |
| NCBI Protein Information: | pleckstrin homology-like domain family A member 3 |
| UniProt Protein Name: | Pleckstrin homology-like domain family A member 3 |
| UniProt Synonym Protein Names: | TDAG51/Ipl homolog 1 |
| Protein Family: | Pleckstrin homology-like domain family |
| UniProt Gene Name: | PHLDA3 |
| UniProt Entry Name: | PHLA3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PHLDA3 antibody (CAB12666) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30min. |
View AllClose