Anti-PFN1 Antibody (CAB1164)
- SKU:
- CAB1164
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PFN1 Antibody |
| 抗体コード: | CAB1164 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat, Monkey |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human PFN1 (NP_005013.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat, Monkey |
| ポジティブサンプル: | Jurkat, HeLa, HT-29, C6, Mouse thymus, Mouse brain, Mouse lung, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human PFN1 (NP_005013.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAGW NAYI DNLM ADGT CQDA AIVG YKDS PSVW AAVP GKTF VNIT PAEV GVLV GKDR SSFY VNGL TLGG QKCS VIRD SLLQ DGEF SMDL RTKS TGGA PTFN VTVT KTDK TLVL LMGK EGVH GGLI NKKC YEMA SHLR RSQY |
| 遺伝子ID: | 5216 |
| Uniprot: | P07737 |
| セルラーロケーション: | Cytoplasm, cytoskeleton |
| 計算された分子量: | 15kDa |
| 観察された分子量: | 13KDa |
| 同義語: | PFN1, ALS18 |
| バックグラウンド: | This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1. |
| UniProt Protein Function: | profilin 1: a ubiquitous actin monomer-binding protein belonging to the profilin family. At high concentrations, profilin 1 prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Deletion of this gene is associated with Miller-Dieker syndrome. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: cell cortex; cytoplasm; cytosol; focal adhesion; membrane; nucleus Molecular Function:actin binding; actin monomer binding; adenyl-nucleotide exchange factor activity; phosphatidylinositol-4,5-bisphosphate binding; protein binding Biological Process: negative regulation of actin filament bundle formation; negative regulation of actin filament polymerization; negative regulation of stress fiber formation; positive regulation of actin filament bundle formation; positive regulation of actin filament polymerization; positive regulation of ATPase activity; protein stabilization; Wnt receptor signaling pathway, planar cell polarity pathway Disease: Amyotrophic Lateral Sclerosis 18 |
| NCBI Summary: | This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1. [provided by RefSeq, Jul 2012] |
| UniProt Code: | P07737 |
| NCBI GenInfo Identifier: | 130979 |
| NCBI Gene ID: | 5216 |
| NCBI Accession: | P07737.2 |
| UniProt Secondary Accession: | P07737,Q53Y44, |
| UniProt Related Accession: | P07737 |
| Molecular Weight: | 15,054 Da |
| NCBI Full Name: | Profilin-1 |
| NCBI Synonym Full Names: | profilin 1 |
| NCBI Official Symbol: | PFN1 |
| NCBI Official Synonym Symbols: | ALS18 |
| NCBI Protein Information: | profilin-1 |
| UniProt Protein Name: | Profilin-1 |
| UniProt Synonym Protein Names: | Epididymis tissue protein Li 184a; Profilin I |
| UniProt Gene Name: | PFN1 |
| UniProt Entry Name: | PROF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PFN1 Polyclonal Antibody (CAB1164) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using PFN1 Polyclonal Antibody (CAB1164) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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