Anti-P4HTM Antibody (CAB6143)
- SKU:
- CAB6143
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-P4HTM Antibody |
| 抗体コード: | CAB6143 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 294-563 of human P4HTM (NP_808807.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, Jurkat, 293T, U-87MG, HeLa, Mouse heart, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 294-563 of human P4HTM (NP_808807.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RQRV LRLT RLSP EIVE LSEP LQVV RYGE GGHY HAHV DSGP VYPE TICS HTKL VANE SVPF ETSC RQVS PNWG LPSI LRPG TPMT QAQP CTVG VPLG MGPG DHWV IPVS PWEH PQLG TCSV PPLP YSYM TVLF YLNN VTGG GETV FPVA DNRT YDEM SLIQ DDVD LRDT RRHC DKGN LRVK PQQG TAVF WYNY LPDG QGWV GDVD DYSL HGGC LVTR GTKW IANN WINV DPSR ARQA LFQQ EMAR LARE GGTD SQPE WALD RAYR DARV EL |
| 遺伝子ID: | 54681 |
| Uniprot: | Q9NXG6 |
| セルラーロケーション: | Endoplasmic reticulum membrane, Single-pass type II membrane protein |
| 計算された分子量: | 47kDa/56kDa/63kDa |
| 観察された分子量: | 55kDa |
| 同義語: | P4HTM, EGLN4, HIFPH4, P4H-TM, PH-4, PH4, PHD4 |
| バックグラウンド: | The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. |
| UniProt Protein Function: | PH4: Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.14.11.-; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p21.31|3p21.3 Cellular Component: endoplasmic reticulum membrane; integral to membrane Molecular Function:L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; calcium ion binding |
| NCBI Summary: | The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NXG6 |
| NCBI GenInfo Identifier: | 32129516 |
| NCBI Gene ID: | 54681 |
| NCBI Accession: | Q9NXG6.2 |
| UniProt Secondary Accession: | Q9NXG6,Q6PAG6, Q8TCJ9, Q8WV55, Q96F22, Q9BW77, |
| UniProt Related Accession: | Q9NXG6 |
| Molecular Weight: | 502 |
| NCBI Full Name: | Transmembrane prolyl 4-hydroxylase |
| NCBI Synonym Full Names: | prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) |
| NCBI Official Symbol: | P4HTM |
| NCBI Official Synonym Symbols: | PH4; PH-4; PHD4; EGLN4; HIFPH4; P4H-TM |
| NCBI Protein Information: | transmembrane prolyl 4-hydroxylase; HPH-4; HIF-PH4; proline 4-hydroxylase; HIF-prolyl hydroxylase 4; P4H with transmembrane domain; Prolyl hydroxlase domain-containing 4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4 |
| UniProt Protein Name: | Transmembrane prolyl 4-hydroxylase |
| UniProt Synonym Protein Names: | Hypoxia-inducible factor prolyl hydroxylase 4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4 |
| Protein Family: | Transmembrane prolyl 4-hydroxylase |
| UniProt Gene Name: | P4HTM |
| UniProt Entry Name: | P4HTM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using P4HTM antibody (CAB6143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
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