Anti-OSBP Antibody (CAB3998)
- SKU:
- CAB3998
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-OSBP Antibody |
| 抗体コード: | CAB3998 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 508-807 of human OSBP (NP_002547.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, HeLa, BT-474, NIH/3T3, Mouse brain, Mouse kidney, Mouse liver, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 508-807 of human OSBP (NP_002547.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LEEN GYRS LCEQ VSHH PPAA AHHA ESKN GWTL RQEI KITS KFRG KYLS IMPL GTIH CIFH ATGH HYTW KKVT TTVH NIIV GKLW IDQS GEID IVNH KTGD KCNL KFVP YSYF SRDV ARKV TGEV TDPS GKVH FALL GTWD EKME CFKV QPVI GENG GDAR QRGH EAEE SRVM LWKR NPLP KNAE NMYY FSEL ALTL NAWE SGTA PTDS RLRP DQRL MENG RWDE ANAE KQRL EEKQ RLSR KKRE AEAM KATE DGTP YDPY KALW FERK KDPV TKEL THIY RGEY WECK EKQD WSSC PDIF |
| 遺伝子ID: | 5007 |
| Uniprot: | P22059 |
| セルラーロケーション: | Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein, cytosol, perinuclear region |
| 計算された分子量: | 89kDa |
| 観察された分子量: | 110kDa |
| 同義語: | OSBP, OSBP1 |
| バックグラウンド: | Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase |
| UniProt Protein Function: | OSBP: Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25- hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability. Belongs to the OSBP family. |
| UniProt Protein Details: | Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 11q12-q13 Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; nucleus; cell junction Molecular Function:protein domain specific binding; protein binding; oxysterol binding Biological Process: lipid transport |
| NCBI Summary: | Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22059 |
| NCBI GenInfo Identifier: | 129308 |
| NCBI Gene ID: | 5007 |
| NCBI Accession: | P22059.1 |
| UniProt Secondary Accession: | P22059,Q6P524, |
| UniProt Related Accession: | P22059 |
| Molecular Weight: | 807 |
| NCBI Full Name: | Oxysterol-binding protein 1 |
| NCBI Synonym Full Names: | oxysterol binding protein |
| NCBI Official Symbol: | OSBP |
| NCBI Official Synonym Symbols: | OSBP1 |
| NCBI Protein Information: | oxysterol-binding protein 1 |
| UniProt Protein Name: | Oxysterol-binding protein 1 |
| Protein Family: | Oxysterol-binding protein |
| UniProt Gene Name: | OSBP |
| UniProt Entry Name: | OSBP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OSBP antibody (CAB3998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of NIH-3T3 cells using OSBP Rabbit pAb (CAB3998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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