Anti-NSMCE1 Antibody (CAB15966)
- SKU:
- CAB15966
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-NSMCE1 Antibody |
| 抗体コード: | CAB15966 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 7-266 of human NSMCE1 (NP_659547.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | BxPC-3, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 7-266 of human NSMCE1 (NP_659547.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RMGV MTDV HRRF LQLL MTHG VLEE WDVK RLQT HCYK VHDR NATV DKLE DFIN NINS VLES LYIE IKRG VTED DGRP IYAL VNLA TTSI SKMA TDFA ENEL DLFR KALE LIID SETG FASS TNIL NLVD QLKG KKMR KKEA EQVL QKFV QNKW LIEK EGEF TLHG RAIL EMEQ YIRE TYPD AVKI CNIC HSLL IQGQ SCET CGIR MHLP CVAK YFQS NAEP RCPH CNDY WPHE IPKV FDPE KERE SGVL KSNK KSLR SRQH |
| 遺伝子ID: | 197370 |
| Uniprot: | Q8WV22 |
| セルラーロケーション: | Chromosome, Nucleus, telomere |
| 計算された分子量: | 30kDa |
| 観察された分子量: | 31kDa |
| 同義語: | NSMCE1, NSE1, NSE1 homolog |
| バックグラウンド: |
| UniProt Protein Function: | NSMCE1: Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT- associated PML bodies (APBs). Has in vitro ubiquitin ligase activity in presence of NDNL2. Is involved in positive regulation of response to DNA damage stimulus. Belongs to the NSE1 family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; EC 6.3.2.- Chromosomal Location of Human Ortholog: 16p12.1 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: double-strand break repair via homologous recombination; postreplication repair; protein sumoylation |
| UniProt Code: | Q8WV22 |
| NCBI GenInfo Identifier: | 209572785 |
| NCBI Gene ID: | 197370 |
| NCBI Accession: | Q8WV22.5 |
| UniProt Secondary Accession: | Q8WV22,Q9P045, Q9P049, D3DWF6, |
| UniProt Related Accession: | Q8WV22 |
| Molecular Weight: | 30,855 Da |
| NCBI Full Name: | Non-structural maintenance of chromosomes element 1 homolog |
| NCBI Synonym Full Names: | NSE1 homolog, SMC5-SMC6 complex component |
| NCBI Official Symbol: | NSMCE1 |
| NCBI Official Synonym Symbols: | NSE1 |
| NCBI Protein Information: | non-structural maintenance of chromosomes element 1 homolog |
| UniProt Protein Name: | Non-structural maintenance of chromosomes element 1 homolog |
| UniProt Gene Name: | NSMCE1 |
| UniProt Entry Name: | NSE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NSMCE1 antibody (CAB15966) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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