Anti-NSFL1C Antibody (CAB6677)
- SKU:
- CAB6677
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NSFL1C Antibody |
| 抗体コード: | CAB6677 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2). |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, U-251MG, Mouse kidney, Mouse brain, Rat brain, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAE RQEA LREF VAVT GAEE DRAR FFLE SAGW DLQI ALAS FYED GGDE DIVT ISQA TPSS VSRG TAPS DNRV TSFR DLIH DQDE DEEE EEGQ RFYA GGSE RSGQ QIVG PPRK KSPN ELVD DLFK GAKE HGAV AVER VTKS PGET SKPR PFAG GGYR LGAA PEEE SAYV AGEK RQHS SQDV HVVL K |
| 遺伝子ID: | 55968 |
| Uniprot: | Q9UNZ2 |
| セルラーロケーション: | Chromosome, Golgi apparatus, Golgi stack, Nucleus |
| 計算された分子量: | 28kDa/37kDa/40kDa |
| 観察された分子量: | 41kDa |
| 同義語: | NSFL1C, P47, UBX1, UBXD10, UBXN2C, dJ776F14.1 |
| バックグラウンド: | N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. |
| UniProt Protein Function: | NSFL1C: Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Belongs to the NSFL1C family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 20p13 Cellular Component: nucleoplasm; intermediate filament cytoskeleton; Golgi stack; cytoplasm; plasma membrane; chromosome; cytosol Molecular Function:ubiquitin binding; protein binding; protein phosphatase type 1 regulator activity; lipid binding Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of catalytic activity; nuclear envelope reassembly; Golgi organization and biogenesis; autophagic vacuole formation |
| NCBI Summary: | N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011] |
| UniProt Code: | Q9UNZ2 |
| NCBI GenInfo Identifier: | 41017512 |
| NCBI Gene ID: | 55968 |
| NCBI Accession: | Q9UNZ2.2 |
| UniProt Secondary Accession: | Q9UNZ2,Q5JXA4, Q5JXA5, Q7Z533, Q9H102, Q9NVL9, Q9UI06 A2A2L1, B2RD74, |
| UniProt Related Accession: | Q9UNZ2 |
| Molecular Weight: | 370 |
| NCBI Full Name: | NSFL1 cofactor p47 |
| NCBI Synonym Full Names: | NSFL1 (p97) cofactor (p47) |
| NCBI Official Symbol: | NSFL1C |
| NCBI Official Synonym Symbols: | P47; UBX1; UBXD10; UBXN2C; dJ776F14.1 |
| NCBI Protein Information: | NSFL1 cofactor p47; SHP1 homolog; p97 cofactor p47; UBX domain-containing protein 2C |
| UniProt Protein Name: | NSFL1 cofactor p47 |
| UniProt Synonym Protein Names: | UBX domain-containing protein 2C; p97 cofactor p47 |
| Protein Family: | NSFL1 cofactor |
| UniProt Gene Name: | NSFL1C |
| UniProt Entry Name: | NSF1C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NSFL1C antibody (CAB6677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using NSFL1C antibody (CAB6677). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody (CAB6677). Western blot was performed from the immunoprecipitate using NSFL1C antibody (CAB6677) at a dilition of 1:1000. |
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