Anti-NFYA Antibody (CAB1998)
- SKU:
- CAB1998
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NFYA Antibody |
| 抗体コード: | CAB1998 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human NFYA (NP_068351.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | HeLa, LO2, BT-474 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human NFYA (NP_068351.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEQY TANS NSST EQIV VQAG QIQQ QVQG QPLM VQVS GGQL ITST GQPI MVQA VPGG QGQT IMQV PVSG TQGL QQIQ LVPP GQIQ IQGG QAVQ VQGQ QGQT QQII IQQP QTAV TAGQ TQTQ QQIA VQGQ QVAQ TAEG QTIV YQPV NADG TILQ QVTV PVSG MITI PAAS LAGA QIVQ TGAN TNTT SSGQ GTVT VTLP VAGN VVNS GGMV MMVP GAGS VPAI QRIP LPGA EMLE EEPL YVNA KQYH RILK RRQA RAKL EAEG KIPK ERRK YLHE SRHR HAMA RKRG EGGR FFSP KEKD SPHM QDPN QADE EAMT QIIR VS |
| 遺伝子ID: | 4800 |
| Uniprot: | P23511 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 33kDa/36kDa |
| 観察された分子量: | 37kDa |
| 同義語: | NFYA, CBF-A, CBF-B, HAP2, NF-YA |
| バックグラウンド: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. |
| UniProt Protein Function: | NFYA: stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. Involved in cisplatin resistance. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: CCAAT-binding factor complex; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P23511 |
| NCBI GenInfo Identifier: | 115844 |
| NCBI Gene ID: | 4800 |
| NCBI Accession: | P23511.2 |
| UniProt Secondary Accession: | P23511,Q8IXU0, |
| UniProt Related Accession: | P23511 |
| Molecular Weight: | 37kDa |
| NCBI Full Name: | Nuclear transcription factor Y subunit alpha |
| NCBI Synonym Full Names: | nuclear transcription factor Y subunit alpha |
| NCBI Official Symbol: | NFYA |
| NCBI Official Synonym Symbols: | HAP2; CBF-A; CBF-B; NF-YA |
| NCBI Protein Information: | nuclear transcription factor Y subunit alpha |
| UniProt Protein Name: | Nuclear transcription factor Y subunit alpha |
| UniProt Synonym Protein Names: | CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A; NF-YA |
| Protein Family: | Nuclear transcription factor |
| UniProt Gene Name: | NFYA |
 | Western blot analysis of extracts of various cell lines, using NFYA antibody (CAB1998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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