Anti-NFKBIB Antibody (CAB5777)
- SKU:
- CAB5777
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NFKBIB Antibody |
| 抗体コード: | CAB5777 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, 293T, Mouse liver, Mouse skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAGV ACLG KAAD ADEW CDSG LGSL GPDA AAPG GPGL GAEL GPGL SWAP LVFG YVTE DGDT ALHL AVIH QHEP FLDF LLGF SAGT EYMD LQND LGQT ALHL AAIL GETS TVEK LYAA GAGL CVAE RRGH TALH LACR VGAH ACAR ALLQ PRPR RPRE APDT YLAQ GPDR TPDT NHTP VALY PDSD LEKE EEES EEDW KLQL EAEN YEGH TPLH VAVI HKDV EMVR LLRD AGAD LDKP EPTC GRSP LHLA VEAQ AADV LELL LRAG ANPA ARMY GGRT PLGS AMLR PNPI LARL LRAH GAPE PEGE DEKS GPCS SSSD SDSG DEGD EYDD IVVH SSRS QTRL PPTP ASKP LPDD PRPV |
| 遺伝子ID: | 4793 |
| Uniprot: | Q15653 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 35kDa/37kDa |
| 観察された分子量: | 40-50kDa |
| 同義語: | NFKBIB, IKBB, TRIP9 |
| バックグラウンド: | The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | IkB-beta: a protein of the NF-kappa-B inhibitor family. Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA- dependent inactivation. Association with inhibitor kappa B- interacting Ras-like proteins 1 and 2 may also explain the slower degradation. Interacts with ligand-binding domain of thyroid hormone receptor-beta, inhibitor kappa B-interacting Ras-like protein 1, inhibitor kappa B-interacting protein Ras2, p65 (RELA) and c-Rel. Expressed in all tissues examined. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: cytosol; nucleus Molecular Function:protein binding; signal transducer activity; transcription coactivator activity Biological Process: transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway |
| NCBI Summary: | The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011] |
| UniProt Code: | Q15653 |
| NCBI GenInfo Identifier: | 57015399 |
| NCBI Gene ID: | 4793 |
| NCBI Accession: | Q15653.2 |
| UniProt Secondary Accession: | Q15653,Q96BJ7, A8K3F4, |
| UniProt Related Accession: | Q15653 |
| Molecular Weight: | 356 |
| NCBI Full Name: | NF-kappa-B inhibitor beta |
| NCBI Synonym Full Names: | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta |
| NCBI Official Symbol: | NFKBIB |
| NCBI Official Synonym Symbols: | IKBB; TRIP9 |
| NCBI Protein Information: | NF-kappa-B inhibitor beta; ikB-B; TRIP-9; ikB-beta; ikappaBbeta; NF-kappa-BIB; I-kappa-B-beta; TR-interacting protein 9; thyroid receptor-interacting protein 9 |
| UniProt Protein Name: | NF-kappa-B inhibitor beta |
| UniProt Synonym Protein Names: | I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9 |
| Protein Family: | NF-kappa-B inhibitor |
| UniProt Gene Name: | NFKBIB |
| UniProt Entry Name: | IKBB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NFKBIB antibody (CAB5777) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat lung using NFKBIB antibody (CAB5777) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using NFKBIB antibody (CAB5777) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human kidney cancer using NFKBIB antibody (CAB5777) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using NFKBIB antibody (CAB5777). Blue: DAPI for nuclear staining. |
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