Anti-MYH10 Antibody (CAB12029)
- SKU:
- CAB12029
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| 抗体名: | Anti-MYH10 Antibody |
| 抗体コード: | CAB12029 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1908-2007 of human MYH10 (NP_001242941.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, NIH/3T3, HT-29, Mouse brain, Mouse kidney, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1908-2007 of human MYH10 (NP_001242941.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ARMK QLKR QLEE AEEE ATRA NASR RKLQ RELD DATE ANEG LSRE VSTL KNRL RRGG PISF SSSR SGRR QLHL EGAS LELS DDDT ESKT SDVN ETQP PQSE |
| 遺伝子ID: | 4628 |
| Uniprot: | P35580 |
| セルラーロケーション: | Cell projection, lamellipodium |
| 計算された分子量: | 228kDa/230kDa/231kDa/232kDa |
| 観察された分子量: | 250kDa |
| 同義語: | MYH10, NMMHC-IIB, NMMHCB, myosin-10 |
| バックグラウンド: | This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MYH10: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with PLEKHG6. Interacts with ECM29. Interacts with KIF26B. Interacts with LARP6. Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 17p13 Cellular Component: mitochondrion; myosin II complex; dendritic spine; actomyosin; cell cortex; actin cytoskeleton; growth cone; cell soma; axon; lamellipodium; cytoplasm; plasma membrane; stress fiber; spindle; midbody; neuromuscular junction; nucleus; myosin complex; cleavage furrow Molecular Function:calmodulin binding; microfilament motor activity; actin filament binding; protein binding; ADP binding; actin-dependent ATPase activity; actin binding; ATP binding Biological Process: adult heart development; axon guidance; exocytosis; metabolic process; actin filament-based movement; in utero embryonic development; fourth ventricle development; ventricular cardiac muscle cell development; actomyosin structure organization and biogenesis; cerebellar Purkinje cell layer development; neuron migration; cardiac myofibril assembly; third ventricle development; substrate-bound cell migration, cell extension; regulation of cell shape; cell proliferation; cytokinesis after mitosis; plasma membrane repair; retina development in camera-type eye; ephrin receptor signaling pathway; neuromuscular process controlling balance; cell adhesion; nuclear migration; lateral ventricle development |
| NCBI Summary: | This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| UniProt Code: | P35580 |
| NCBI GenInfo Identifier: | 215274129 |
| NCBI Gene ID: | 4628 |
| NCBI Accession: | P35580.3 |
| UniProt Related Accession: | P35580 |
| Molecular Weight: | 250kDa |
| NCBI Full Name: | Myosin-10 |
| NCBI Synonym Full Names: | myosin heavy chain 10 |
| NCBI Official Symbol: | MYH10 |
| NCBI Official Synonym Symbols: | NMMHCB; NMMHC-IIB |
| NCBI Protein Information: | myosin-10 |
| UniProt Protein Name: | Myosin-10 |
| UniProt Synonym Protein Names: | Cellular myosin heavy chain, type B; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; Non-muscle myosin heavy chain B; NMMHC-B; Non-muscle myosin heavy chain IIb; NMMHC II-b; NMMHC-IIB |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYH10 |
| UniProt Entry Name: | MYH10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYH10 antibody (CAB12029) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using MYH10 antibody (CAB12029) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using MYH10 antibody (CAB12029) at dilution of 1:100 (40x lens). |