Anti-MRPS14 Antibody (CAB15498)
- SKU:
- CAB15498
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-MRPS14 Antibody |
| 抗体コード: | CAB15498 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human MRPS14 (NP_071383.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | BxPC-3, U-251MG, Mouse heart, Mouse kidney, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human MRPS14 (NP_071383.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAF MLGS LLRT FKQM VPSS ASGQ VRSH YVDW RMWR DVKR RKMA YEYA DERL RINS LRKN TILP KILQ DVAD EEIA ALPR DSCP VRIR NRCV MTSR PRGV KRRW RLSR IVFR HLAD HGQL SGIQ RATW |
| 遺伝子ID: | 63931 |
| Uniprot: | O60783 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 15kDa |
| 観察された分子量: | 15-17kDa |
| 同義語: | MRPS14, DJ262D12.2, HSMRPS14, MRP-S14, S14mt |
| バックグラウンド: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] |
| UniProt Code: | O60783 |
| NCBI GenInfo Identifier: | 11559927 |
| NCBI Gene ID: | 63931 |
| NCBI Accession: | NP_071383.1 |
| UniProt Secondary Accession: | O60783,Q5R358, |
| UniProt Related Accession: | O60783 |
| Molecular Weight: | 14 |
| NCBI Full Name: | 28S ribosomal protein S14, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein S14 |
| NCBI Official Symbol: | MRPS14 |
| NCBI Official Synonym Symbols: | S14mt; MRP-S14; HSMRPS14; DJ262D12.2 |
| NCBI Protein Information: | 28S ribosomal protein S14, mitochondrial |
| UniProt Protein Name: | 28S ribosomal protein S14, mitochondrial |
| UniProt Synonym Protein Names: | Mitochondrial small ribosomal subunit protein uS14m |
| UniProt Gene Name: | MRPS14 |
 | Western blot analysis of extracts of various cell lines, using MRPS14 antibody (CAB15498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa cells using MRPS14 antibody (CAB15498) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using MRPS14 antibody (CAB15498) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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