Anti-MGMT Antibody (RACO0289)
- SKU:
- RACO0289
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | MGMT Antibody |
| Product SKU: | RACO0289 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human MGMT |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| シノニム: | Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase), MGMT |
 | IHC image of RACO0289 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in MCF-7 whole cell lysate Jurkat whole cell lysate All lanes MGMT antibody at 1:1500. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 22 kDa. Observed band size: 22 kDa. |
| UniProt Protein Function: | MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family. |
| UniProt Protein Details: | Protein type:EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis Chromosomal Location of Human Ortholog: 10q26 Cellular Component: nucleoplasm; membrane; nucleus Molecular Function:methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation |
| UniProt Code: | P16455 |
| NCBI GenInfo Identifier: | 127069 |
| NCBI Gene ID: | 4255 |
| NCBI Accession: | P16455.1 |
| UniProt Secondary Accession: | P16455,Q5VY78, |
| UniProt Related Accession: | P16455 |
| Molecular Weight: | 21,646 Da |
| NCBI Full Name: | Methylated-DNA--protein-cysteine methyltransferase |
| NCBI Synonym Full Names: | O-6-methylguanine-DNA methyltransferase |
| NCBI Official Symbol: | MGMT |
| NCBI Protein Information: | methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase |
| UniProt Protein Name: | Methylated-DNA--protein-cysteine methyltransferase |
| UniProt Synonym Protein Names: | 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase |
| Protein Family: | Probable methylated-DNA--protein-cysteine methyltransferase |
| UniProt Gene Name: | MGMT |
| UniProt Entry Name: | MGMT_HUMAN |