Anti-ME2 Antibody (CAB9650)
- SKU:
- CAB9650
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ME2 Antibody |
| 抗体コード: | CAB9650 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-479 of human ME2 (NP_001161807.1). |
| 申し込み: | WB IP |
| 推奨希釈: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | LO2, HL-60, SKOV3, HT-1080 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-479 of human ME2 (NP_001161807.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GLYQ KRDR TQQY DDLI DEFM KAIT DRYG RNTL IQFE DFGN HNAF RFLR KYRE KYCT FNDD IQGT AAVA LAGL LAAQ KVIS KPIS EHKI LFLG AGEA ALGI ANLI VMSM VENG LSEQ EAQK KIWM FDKY GLLV KGRK AKID SYQE PFTH SAPE SIPD TFED AVNI LKPS TIIG VAGA GRLF TPDV IRAM ASIN ERPV IFAL SNPT AQAE CTAE EAYT LTEG RCLF ASGS PFGP VKLT DGRV FTPG QGNN VYIF PGYR IPIC |
| 遺伝子ID: | 4200 |
| Uniprot: | P23368 |
| セルラーロケーション: | Mitochondrion matrix |
| 計算された分子量: | 53kDa/65kDa |
| 観察された分子量: | 59kDa/65kDa |
| 同義語: | ME2, ODS1 |
| バックグラウンド: | This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. |
| UniProt Protein Function: | ME2: a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009] |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pyruvate; Oxidoreductase; Mitochondrial; EC 1.1.1.38 Chromosomal Location of Human Ortholog: 18q21 Cellular Component: intracellular membrane-bound organelle; mitochondrion Molecular Function:electron carrier activity; malic enzyme activity Disease: Epilepsy, Idiopathic Generalized; Opioid Dependence, Susceptibility To, 1 |
| NCBI Summary: | This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009] |
| UniProt Code: | P23368 |
| NCBI GenInfo Identifier: | 126733 |
| NCBI Gene ID: | 4200 |
| NCBI Accession: | P23368.1 |
| UniProt Secondary Accession: | P23368,Q9BWL6, Q9BYG1, Q9H4B2, B2R8J2, |
| UniProt Related Accession: | P23368 |
| Molecular Weight: | 53,586 Da |
| NCBI Full Name: | NAD-dependent malic enzyme, mitochondrial |
| NCBI Synonym Full Names: | malic enzyme 2 |
| NCBI Official Symbol: | ME2 |
| NCBI Official Synonym Symbols: | ODS1 |
| NCBI Protein Information: | NAD-dependent malic enzyme, mitochondrial |
| UniProt Protein Name: | NAD-dependent malic enzyme, mitochondrial |
| UniProt Synonym Protein Names: | Malic enzyme 2 |
| UniProt Gene Name: | ME2 |
| UniProt Entry Name: | MAOM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ME2 antibody (CAB9650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug ME2 antibody (CAB9650). Western blot was performed from the immunoprecipitate using ME2 antibody (CAB9650) at a dilition of 1:1000. |
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