Anti-MAPKAPK2 Antibody (RACO0482)
- SKU:
- RACO0482
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | MAPKAPK2 Antibody |
| Product SKU: | RACO0482 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human MAPKAP Kinase 2 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed:16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed:26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. |
| シノニム: | MAP kinase-activated protein kinase 2 (MAPK-activated protein kinase 2) (MAPKAP kinase 2) (MAPKAP-K2) (MAPKAPK-2) (MK-2) (MK2) (EC 2.7.11.1), MAPKAPK2 |
 | IHC image of RACO0482 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | IHC image of RACO0482 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Hela whole cell lysate HEK293 whole cell lysate THP-1 whole cell lysate All lanes MAPKAPK2 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 46, 43 kDa. Observed band size: 49 kDa. |
| UniProt Protein Function: | MAPKAPK2: a member of the MAPKAPK family of protein kinases. It is phosphorylated and activated by p38 in response to cytokines, stress and chemotactic factors.MAPKAPK-2 is apparently a direct target of p38 MAPK. HSP27 is one of its in vivo substrates. Two transcript variants encoding two different isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; MAPKAPK family; MAPKAPK subfamily Chromosomal Location of Human Ortholog: 1q32 Cellular Component: centrosome; cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:ATP binding; calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; signal transducer activity Biological Process: activation of MAPK activity; G2/M transition DNA damage checkpoint; inflammatory response; inner ear development; leukotriene metabolic process; MAPKKK cascade; peptidyl-serine phosphorylation; positive regulation of tumor necrosis factor biosynthetic process; protein amino acid autophosphorylation; protein amino acid phosphorylation; Ras protein signal transduction; regulation of interleukin-6 production; regulation of mRNA stability; regulation of tumor necrosis factor production; response to cytokine stimulus; response to DNA damage stimulus; response to lipopolysaccharide; response to stress; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49137 |
| NCBI GenInfo Identifier: | 1346538 |
| NCBI Gene ID: | 9261 |
| NCBI Accession: | P49137.1 |
| UniProt Secondary Accession: | P49137,Q5SY30, Q5SY41, Q8IYD6, |
| UniProt Related Accession: | P49137 |
| Molecular Weight: | 42,203 Da |
| NCBI Full Name: | MAP kinase-activated protein kinase 2 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase-activated protein kinase 2 |
| NCBI Official Symbol: | MAPKAPK2 |
| NCBI Official Synonym Symbols: | MK2; MK-2; MAPKAP-K2 |
| NCBI Protein Information: | MAP kinase-activated protein kinase 2 |
| UniProt Protein Name: | MAP kinase-activated protein kinase 2 |
| UniProt Gene Name: | MAPKAPK2 |
| UniProt Entry Name: | MAPK2_HUMAN |