Anti-MAPK8IP1 Antibody (CAB9011)
- SKU:
- CAB9011
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MAPK8IP1 Antibody |
| 抗体コード: | CAB9011 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MAPK8IP1 (NP_005447.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:200 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | U-87MG, HT-1080, SKOV3, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MAPK8IP1 (NP_005447.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAER ESGG LGGG AASP PAAS PFLG LHIA SPPN FRLT HDIS LEEF EDED LSEI TDEC GISL QCKD TLSL RPPR AGLL SAGG GGAG SRLQ AEML QMDL IDAT GDTP GAED DEED DDEE RAAR RPGA GPPK AESG QEPA SRGQ GQSQ GQSQ GPGS GDTY RPKR PTTL NLFP QVPR SQDT LNNN SLGK KHSW QDRV SRSS SPLK TGEQ TPPH EHIC LSDE LPPQ SGPA PTTD RGTS TDSP CRRS TATQ MAPP GG |
| 遺伝子ID: | 9479 |
| Uniprot: | Q9UQF2 |
| セルラーロケーション: | Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion membrane, Nucleus, perinuclear region |
| 計算された分子量: | 77kDa |
| 観察された分子量: | 78kDa |
| 同義語: | MAPK8IP1, IB1, JIP-1, JIP1, PRKM8IP |
| バックグラウンド: | This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. |
| UniProt Protein Function: | JIP1: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade. Required for JNK activation in response to excitotoxic stress. Causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and by inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly or indirectly regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: cytoplasm Molecular Function:kinesin binding; MAP-kinase scaffold activity; protein binding; protein kinase inhibitor activity Biological Process: regulation of JNK cascade; vesicle-mediated transport Disease: Diabetes Mellitus, Noninsulin-dependent |
| NCBI Summary: | This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011] |
| UniProt Code: | Q9UQF2 |
| NCBI GenInfo Identifier: | 17433093 |
| NCBI Gene ID: | 9479 |
| NCBI Accession: | Q9UQF2.1 |
| UniProt Secondary Accession: | Q9UQF2,O43407, D3DQP4, |
| UniProt Related Accession: | Q9UQF2 |
| Molecular Weight: | 77,524 Da |
| NCBI Full Name: | C-Jun-amino-terminal kinase-interacting protein 1 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase 8 interacting protein 1 |
| NCBI Official Symbol: | MAPK8IP1 |
| NCBI Official Synonym Symbols: | IB1; JIP1; JIP-1; PRKM8IP |
| NCBI Protein Information: | C-Jun-amino-terminal kinase-interacting protein 1 |
| UniProt Protein Name: | C-Jun-amino-terminal kinase-interacting protein 1 |
| UniProt Synonym Protein Names: | Islet-brain 1; IB-1; JNK MAP kinase scaffold protein 1; Mitogen-activated protein kinase 8-interacting protein 1 |
| Protein Family: | C-Jun-amino-terminal kinase-interacting protein |
| UniProt Gene Name: | MAPK8IP1 |
| UniProt Entry Name: | JIP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAPK8IP1 antibody (CAB9011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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