Anti-macroH2A.1 Antibody (CAB18091)[KO Validated]
![Anti-macroH2A.1 Antibody (CAB18091)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/14396/14283/anti-macroh2a.1-antibody-cab18091ko-validated__17856__50475.1645802653.jpg?c=1 "Anti-macroH2A.1 Antibody (CAB18091)[KO Validated]")
- SKU:
- CAB18091
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
![Anti-macroH2A.1 Antibody (CAB18091)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/14396/14283/anti-macroh2a.1-antibody-cab18091ko-validated__17856__50475.1645802653.jpg?c=1&imbypass=on "Anti-macroH2A.1 Antibody (CAB18091)[KO Validated]")
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Description
| 抗体名: | Anti-macroH2A.1 Antibody [KO Validated] |
| 抗体コード: | CAB18091 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human macroH2A.1 (NP_613258.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human macroH2A.1 (NP_613258.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KLEA IITP PPAK KAKS PSQK KPVS KKAG GKKG ARKS KKKQ GEVS KAAS ADST TEGT PADG FTVL STKS LFLG QKLN LIHS EISN LAGF EVEA IINP TNAD IDLK DDLG NTLE KKGG KEFV EAVL ELRK KNGP LEVA GAAV SAGH GLPA KFVI HCNS PVWG ADKC EELL EKTV KNCL ALAD DKKL KSIA FPSI GSGR NGFP KQTA AQLI LKAI SSYF VSTM SSSI KTVY FVLF DSES IGIY VQEM AKLD AN |
| 遺伝子ID: | 9555 |
| Uniprot: | O75367 |
| セルラーロケーション: | Chromosome, Nucleus |
| 計算された分子量: | 39kDa |
| 観察された分子量: | 40kDa |
![Anti-macroH2A.1 Antibody [KO Validated] (CAB18091)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/q/063/A18091_1__72355.jpg) | Western blot analysis of extracts from normal (control) and macroH2A.1 knockout (KO) 293T cells, using H2AFY antibody (CAB18091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
| 同義語: | H2AFY, H2A.y, H2A/y, H2AF12M, MACROH2A1.1, mH2A1, macroH2A1.2 |
| バックグラウンド: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | H2AFY: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: nucleoplasm; centric heterochromatin; sex chromatin; ESC/E(Z) complex; nuclear chromatin; nucleolus; Barr body; condensed chromosome; nucleosome; nucleus Molecular Function:rDNA binding; protein serine/threonine kinase inhibitor activity; protein binding; DNA binding; double-stranded methylated DNA binding; chromatin DNA binding; protein heterodimerization activity; protein kinase binding Biological Process: nucleosome assembly; negative regulation of gene expression, epigenetic; dosage compensation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of cell growth; negative regulation of histone phosphorylation |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75367 |
| NCBI GenInfo Identifier: | 90110023 |
| NCBI Gene ID: | 9555 |
| NCBI Accession: | O75367.4 |
| UniProt Secondary Accession: | O75367,O75377, Q503A8, Q7Z5E3, Q96D41, Q9H8P3, Q9UP96 |
| UniProt Related Accession: | O75367 |
| Molecular Weight: | 372 |
| NCBI Full Name: | Core histone macro-H2A.1 |
| NCBI Synonym Full Names: | H2A histone family, member Y |
| NCBI Official Symbol: | H2AFY |
| NCBI Official Synonym Symbols: | H2A.y; H2A/y; H2AFJ; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2 |
| NCBI Protein Information: | core histone macro-H2A.1; histone H2A.y; histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; medulloblastoma antigen MU-MB-50.205 |
| UniProt Protein Name: | Core histone macro-H2A.1 |
| UniProt Synonym Protein Names: | Histone H2A.y; H2A/y; Medulloblastoma antigen MU-MB-50.205 |
| Protein Family: | Core histone macro |
| UniProt Gene Name: | H2AFY |
| UniProt Entry Name: | H2AY_HUMAN |