Anti-LFNG Antibody (CAB7441)
- SKU:
- CAB7441
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-LFNG Antibody |
| 抗体コード: | CAB7441 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human LFNG (NP_002295.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, SW620, PC-12, HeLa, Mouse spleen, Mouse brain, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human LFNG (NP_002295.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MTPG RCCL AADI QVET FIFT DGED EALA RHTG NVVI TNCS AAHS RQAL SCKM AVEY DRFI ESGR KWFC HVDD DNYV NLRA LLRL LASY PHTR DVYV GKPS LDRP IQAM ERVS ENKV RPVH FWFA TGGA GFCI SRGL ALKM SPWA SGGH FMNT AERI RLPD DCTI GYIV EALL GVPL IRSG LFHS HLEN LQQV PTSE LHEQ VTLS YGMF ENKR NAVH VKGP FSVE ADPS RFRS IHCH LYPD TPWC PRTA IF |
| 遺伝子ID: | 3955 |
| Uniprot: | Q8NES3 |
| セルラーロケーション: | Golgi apparatus membrane, Single-pass type II membrane protein |
| 計算された分子量: | 28kDa/35kDa/39kDa/41kDa |
| 観察された分子量: | 42kDa |
| 同義語: | LFNG, SCDO3, beta-1 |
| バックグラウンド: | This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1, 3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | LFNG: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning. Defects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3). An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. Belongs to the glycosyltransferase 31 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Transferase; Membrane protein, integral; EC 2.4.1.222 Chromosomal Location of Human Ortholog: 7p22.2 Cellular Component: extracellular region; integral to Golgi membrane; vesicle Molecular Function:metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity Biological Process: compartment specification; somitogenesis; organ morphogenesis; Notch signaling pathway; regulation of Notch signaling pathway; positive regulation of protein binding; ovarian follicle development; metabolic process; female meiosis; regulation of somitogenesis; positive regulation of Notch signaling pathway Disease: Spondylocostal Dysostosis 3, Autosomal Recessive |
| NCBI Summary: | This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q8NES3 |
| NCBI GenInfo Identifier: | 27734417 |
| NCBI Gene ID: | 3955 |
| NCBI Accession: | Q8NES3.2 |
| UniProt Secondary Accession: | Q8NES3,O00589, Q96C39, Q9UJW5, B3KTY6, B5MCR5, |
| UniProt Related Accession: | Q8NES3 |
| Molecular Weight: | 379 |
| NCBI Full Name: | Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe |
| NCBI Synonym Full Names: | LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| NCBI Official Symbol: | LFNG |
| NCBI Official Synonym Symbols: | SCDO3 |
| NCBI Protein Information: | beta-1,3-N-acetylglucosaminyltransferase lunatic fringe |
| UniProt Protein Name: | Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe |
| UniProt Synonym Protein Names: | O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Protein Family: | Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe |
| UniProt Gene Name: | LFNG |
| UniProt Entry Name: | LFNG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LFNG antibody (CAB7441) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using LFNG antibody (CAB7441) at dilution of 1:100 (40x lens). |
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