Anti-ISL1 Antibody (RACO0451)
- SKU:
- RACO0451
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | ISL1 Antibody |
| Product SKU: | RACO0451 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Islet1 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. |
| シノニム: | Insulin gene enhancer protein ISL-1 (Islet-1), ISL1 |
 | IHC image of RACO0451 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | IHC image of RACO0451 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in 293T whole cell lysate K562 whole cell lysate THP-1 whole cell lysate All lanes Islet1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 40 kDa. Observed band size: 40 kDa. |
| UniProt Protein Function: | ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Nuclear receptor co-regulator; DNA-binding; Transcription factor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 5q11.1 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus Molecular Function:ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of estrogen receptor signaling pathway; pancreas development; positive regulation of cell proliferation; ventricular cardiac muscle morphogenesis; neuron fate specification; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; spinal cord motor neuron differentiation; positive regulation of tumor necrosis factor production; positive regulation of interleukin-1 beta production; positive regulation of interferon-gamma production; negative regulation of neuron differentiation; positive regulation of angiogenesis; mesenchymal cell differentiation; visceral motor neuron differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance |
| NCBI Summary: | This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61371 |
| NCBI GenInfo Identifier: | 47606423 |
| NCBI Gene ID: | 3670 |
| NCBI Accession: | P61371.1 |
| UniProt Secondary Accession: | P61371,P20663, P47894, |
| UniProt Related Accession: | P61371 |
| Molecular Weight: | 349 |
| NCBI Full Name: | Insulin gene enhancer protein ISL-1 |
| NCBI Synonym Full Names: | ISL LIM homeobox 1 |
| NCBI Official Symbol: | ISL1 |
| NCBI Official Synonym Symbols: | Isl-1; ISLET1 |
| NCBI Protein Information: | insulin gene enhancer protein ISL-1; islet-1; ISL1 transcription factor, LIM/homeodomain |
| UniProt Protein Name: | Insulin gene enhancer protein ISL-1 |
| Protein Family: | Insulin gene enhancer protein |
| UniProt Gene Name: | ISL1 |
| UniProt Entry Name: | ISL1_HUMAN |