Anti-INPP5B Antibody (CAB3927)
- SKU:
- CAB3927
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-INPP5B Antibody |
| 抗体コード: | CAB3927 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human INPP5B (NP_005531.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Raji, SGC7901, HepG2, Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human INPP5B (NP_005531.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDQS VAIQ ETLA EGEY CVIA VQGV LCEG DSRQ SRLL GLVR YRLE HGGQ EHAL FLYT HRRM AITG DDVS LDQI VPVS RDFT LEEV SPDG ELYI LGSD VTVQ LDTA ELSL VFQL PFGS QTRM FLHE VARA CPGF DSAT RDPE FLWL SRYR CAEL ELEM PTPR GCNS ALVT WPGY ATIG GGGS NFDG LRPN GKGV PMDQ SSRG QDKP ESLQ PRQN KSKS EITD MVRS STIT VSDK AHIL SMQK FGLR DTIV KSHL LQKE EDYT |
| 遺伝子ID: | 3633 |
| Uniprot: | P32019 |
| セルラーロケーション: | Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Early endosome membrane, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Membrane, Peripheral membrane protein, cytosol, phagosome membrane |
| 計算された分子量: | 85kDa/93kDa/103kDa/112kDa |
| 観察された分子量: | 113kDa |
| 同義語: | INPP5B, 5PTase |
| バックグラウンド: | This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. |
| UniProt Protein Function: | INPP5B: Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.36; Phosphatase (non-protein); Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p34 Cellular Component: Golgi apparatus; phagocytic vesicle membrane; membrane; early endosome membrane; cytoplasm; integral to membrane; plasma membrane; ER-Golgi intermediate compartment; cytosol Molecular Function:protein binding; metal ion binding; phosphoinositide 5-phosphatase activity Biological Process: sperm motility; regulation of small GTPase mediated signal transduction; inositol phosphate metabolic process; in utero embryonic development; small GTPase mediated signal transduction; phosphoinositide dephosphorylation; spermatogenesis |
| NCBI Summary: | This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P32019 |
| NCBI GenInfo Identifier: | 37590185 |
| NCBI Gene ID: | 3633 |
| NCBI Accession: | BC058932 |
| UniProt Secondary Accession: | P32019,Q5VSG9, Q5VSH0, Q5VSH1, Q658Q5, Q6P6D4, Q6PD53 Q86YE1, C9J6U5, |
| UniProt Related Accession: | P32019 |
| Molecular Weight: | Calculated MW: 113 kDaObserved MW: 75 kDa |
| NCBI Full Name: | INPP5B protein |
| NCBI Synonym Full Names: | inositol polyphosphate-5-phosphatase, 75kDa |
| NCBI Official Symbol: | INPP5B |
| NCBI Official Synonym Symbols: | 5PTase |
| NCBI Protein Information: | type II inositol 1,4,5-trisphosphate 5-phosphatase; phosphoinositide 5-phosphatase |
| UniProt Protein Name: | Type II inositol 1,4,5-trisphosphate 5-phosphatase |
| UniProt Synonym Protein Names: | 75 kDa inositol polyphosphate-5-phosphatase; Phosphoinositide 5-phosphatase; 5PTase |
| Protein Family: | Type II inositol 1,4,5-trisphosphate 5-phosphatase |
| UniProt Gene Name: | INPP5B |
| UniProt Entry Name: | I5P2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using INPP5B antibody (CAB3927) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of C6 cells using INPP5B antibody (CAB3927) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using INPP5B antibody (CAB3927) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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