Anti-ILF3 Antibody (RACO0506)
- SKU:
- RACO0506
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | ILF3 Antibody |
| Product SKU: | RACO0506 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, IHC, IF |
| 推奨される希釈: | IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human ILF3 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication (By similarity). |
| シノニム: | Interleukin enhancer-binding factor 3 (Double-stranded RNA-binding protein 76) (DRBP76) (M-phase phosphoprotein 4) (MPP4) (Nuclear factor associated with dsRNA) (NFAR) (Nuclear factor of activated T-cells 90 kDa) (NF-AT-90) (Translational control protein 80) (TCP80), ILF3, DRBF MPHOSPH4 NF90 |
 | IHC image of RACO0506 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Immunofluorescence staining of Hela Cells with RACO0506 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | IHC image of RACO0506 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression. Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:RNA-binding; Transcription factor; DNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus Molecular Function:protein binding; DNA binding; RNA binding; double-stranded RNA binding Biological Process: transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation |
| UniProt Code: | Q12906 |
| NCBI GenInfo Identifier: | 62512150 |
| NCBI Gene ID: | |
| NCBI Accession: | Q12906.3 |
| Molecular Weight: | |
| NCBI Full Name: | Interleukin enhancer-binding factor 3 |
| UniProt Protein Name: | Interleukin enhancer-binding factor 3 |
| UniProt Synonym Protein Names: | Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4; Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80 |
| Protein Family: | Interleukin enhancer-binding factor |
| UniProt Gene Name: | ILF3 |
| UniProt Entry Name: | ILF3_HUMAN |