Anti-IFIT1 Antibody (CAB8551)
- SKU:
- CAB8551
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-IFIT1 Antibody |
| 抗体コード: | CAB8551 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 301-388 of human IFIT1 (NP_001539.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:200 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse heart, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 301-388 of human IFIT1 (NP_001539.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QIKE ATKG QPRG QNRE KLDK MIRS AIFH FESA VEKK PTFE VAHL DLAR MYIE AGNH RKAE ENFQ KLLC MKPV VEET MQDI HFHY GRFQ |
| 遺伝子ID: | 3434 |
| Uniprot: | P09914 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 51kDa/55kDa |
| 観察された分子量: | 48kDa |
| 同義語: | IFIT1, C56, G10P1, IFI-56, IFI-56K, IFI56, IFIT-1, IFNAI1, ISG56, P56, RNM561 |
| バックグラウンド: | This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed. |
| UniProt Protein Function: | IFIT1: IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O- methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses. Belongs to the IFIT family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 10q23.31 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding; RNA binding Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; intracellular transport of viral proteins in host cell; negative regulation of defense response to virus by host; negative regulation of helicase activity; negative regulation of protein binding; negative regulation of viral genome replication; positive regulation of viral genome replication; response to virus |
| NCBI Summary: | This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012] |
| UniProt Code: | P09914 |
| NCBI GenInfo Identifier: | 400286381 |
| NCBI Gene ID: | 3434 |
| NCBI Accession: | NP_001257857.1 |
| UniProt Secondary Accession: | P09914,Q5T7J1, Q96QM5, B3KS50, D3DR31, |
| UniProt Related Accession: | P09914 |
| Molecular Weight: | 51,713 Da |
| NCBI Full Name: | interferon-induced protein with tetratricopeptide repeats 1 isoform 3 |
| NCBI Synonym Full Names: | interferon induced protein with tetratricopeptide repeats 1 |
| NCBI Official Symbol: | IFIT1 |
| NCBI Official Synonym Symbols: | C56; P56; G10P1; IFI56; ISG56; IFI-56; IFIT-1; IFNAI1; RNM561; IFI-56K |
| NCBI Protein Information: | interferon-induced protein with tetratricopeptide repeats 1 |
| UniProt Protein Name: | Interferon-induced protein with tetratricopeptide repeats 1 |
| UniProt Synonym Protein Names: | Interferon-induced 56 kDa protein; IFI-56K; P56 |
| Protein Family: | Interferon-induced protein with tetratricopeptide repeats |
| UniProt Gene Name: | IFIT1 |
| UniProt Entry Name: | IFIT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IFIT1 antibody (CAB8551) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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