Anti-IER3 Antibody (CAB8566)
- SKU:
- CAB8566
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-IER3 Antibody |
| 抗体コード: | CAB8566 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human IER3 (NP_003888.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HT-1080, MCF7, SW480, HeLa, A-431 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human IER3 (NP_003888.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GRPS ASRG HRKR SRRV LYPR VVRR QLPV EEPN PAKR LLFL LLTI VFCQ ILMA EEGV PAPL PPED APNA ASLA PTPV SAVL EPFN LTSE PSDY ALDL STFL QQHP AAF |
| 遺伝子ID: | 8870 |
| Uniprot: | P46695 |
| セルラーロケーション: | Membrane, Single-pass type II membrane protein |
| 計算された分子量: | 16kDa |
| 観察された分子量: | 17kDa |
| 同義語: | IER3, DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1 |
| バックグラウンド: | This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. |
| UniProt Protein Function: | IEX-1: May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. Belongs to the IER3 family. |
| UniProt Protein Details: | Protein type:Apoptosis; Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cytosol; nucleus Molecular Function:protein binding Biological Process: anatomical structure morphogenesis; apoptosis; regulation of phosphoinositide 3-kinase cascade |
| NCBI Summary: | This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P46695 |
| NCBI GenInfo Identifier: | 317373569 |
| NCBI Gene ID: | 8870 |
| NCBI Accession: | P46695.4 |
| UniProt Secondary Accession: | P46695,Q5SU30, Q92691, Q93044, |
| UniProt Related Accession: | P46695 |
| Molecular Weight: | 16,903 Da |
| NCBI Full Name: | Radiation-inducible immediate-early gene IEX-1 |
| NCBI Synonym Full Names: | immediate early response 3 |
| NCBI Official Symbol: | IER3 |
| NCBI Official Synonym Symbols: | DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L |
| NCBI Protein Information: | radiation-inducible immediate-early gene IEX-1 |
| UniProt Protein Name: | Radiation-inducible immediate-early gene IEX-1 |
| UniProt Synonym Protein Names: | Differentiation-dependent gene 2 protein; Protein DIF-2; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein; Protein PRG1 |
| Protein Family: | Radiation-inducible immediate-early gene |
| UniProt Gene Name: | IER3 |
| UniProt Entry Name: | IEX1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IER3 Antibody (CAB8566) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse stomach using IER3 antibody (CAB8566) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using IER3 antibody (CAB8566) at dilution of 1:100 (40x lens). |
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