Anti-ID1 Antibody (RACO0541)
- SKU:
- RACO0541
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
| 商品名: | ID1 Antibody |
| Product SKU: | RACO0541 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IF |
| 推奨される希釈: | WB:1:500-1:5000, IF:1:20-1:200 |
| 反応性: | Human, Mouse |
| 免疫原: | A synthesized peptide derived from human Id1 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). |
| シノニム: | DNA-binding protein inhibitor ID-1 (Class B basic helix-loop-helix protein 24) (bHLHb24) (Inhibitor of DNA binding 1) (Inhibitor of differentiation 1), ID1, BHLHB24 ID |
 | Immunofluorescence staining of Hela Cells with RACO0541 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in 293T whole cell lysate Hela whole cell lysate HepG2 whole cell lysate 293 whole cell lysate A549 whole cell lysate U-251 whole cell lysate Mouse Brain whole cell lysate All lanes Id1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 17, 16 kDa. Observed band size: 26 kDa. |
| UniProt Protein Function: | ID1: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; DNA-binding Chromosomal Location of Human Ortholog: 20q11 Cellular Component: nucleoplasm; Golgi apparatus; nucleus Molecular Function:protein dimerization activity; protein binding; transcription factor activity Biological Process: circadian rhythm; collagen metabolic process; blood vessel endothelial cell migration; transcription, DNA-dependent; protein destabilization; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; BMP signaling pathway; response to antibiotic; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; blood vessel morphogenesis; negative regulation of osteoblast differentiation; endothelial cell morphogenesis; angiogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis |
| NCBI Summary: | The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P41134 |
| NCBI GenInfo Identifier: | 31317297 |
| NCBI Gene ID: | 3397 |
| NCBI Accession: | NP_851998.1 |
| UniProt Secondary Accession: | P41134,O00651, O00652, Q16371, Q16377, Q5TE66, Q5TE67 Q969Z7, Q9H0Z5, Q9H109, A8K537, E1P5L4, |
| UniProt Related Accession: | P41134 |
| Molecular Weight: | 15,586 Da |
| NCBI Full Name: | DNA-binding protein inhibitor ID-1 isoform b |
| NCBI Synonym Full Names: | inhibitor of DNA binding 1, HLH protein |
| NCBI Official Symbol: | ID1 |
| NCBI Official Synonym Symbols: | ID; bHLHb24 |
| NCBI Protein Information: | DNA-binding protein inhibitor ID-1 |
| UniProt Protein Name: | DNA-binding protein inhibitor ID-1 |
| UniProt Synonym Protein Names: | Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; Inhibitor of differentiation 1 |
| Protein Family: | DNA-binding protein inhibitor |
| UniProt Gene Name: | ID1 |
| UniProt Entry Name: | ID1_HUMAN |