Anti-ICK Antibody (CAB7783)
- SKU:
- CAB7783
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ICK Antibody |
| 抗体コード: | CAB7783 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ICK (NP_057597.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, SW620, Mouse liver, Mouse lung, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ICK (NP_057597.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MNRY TTIR QLGD GTYG SVLL GRSI ESGE LIAI KKMK RKFY SWEE CMNL REVK SLKK LNHA NVVK LKEV IREN DHLY FIFE YMKE NLYQ LIKE RNKL FPES AIRN IMYQ ILQG LAFI HKHG FFHR DLKP ENLL CMGP ELVK IADF GLAR EIRS KPPY TDYV STRW YRAP EVLL RSTN YSSP IDVW AVGC IMAE VYTL RPLF PGAS EIDT IFKI CQVL GTPK KTDW PEGY QLSS AMNF RWPQ CVPN NLKT LIPN ASSE AVQL LRDM LQWD PK |
| 遺伝子ID: | 22858 |
| Uniprot: | Q9UPZ9 |
| セルラーロケーション: | Cell projection, Cytoplasm, Nucleus, cilium, cilium basal body, cytoskeleton, cytosol |
| 計算された分子量: | 34kDa/71kDa |
| 観察された分子量: | 71kDa |
| 同義語: | ICK, ECO, LCK2, MRK |
| バックグラウンド: | Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. |
| UniProt Protein Function: | Required for ciliogenesis (PubMed:24797473). Phosphorylates KIF3A. Involved in the control of ciliary length (PubMed:24853502). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips. May play a key role in the development of multiple organ systems and particularly in cardiac development. Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity. |
| NCBI Summary: | Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UPZ9 |
| NCBI GenInfo Identifier: | 7662388 |
| NCBI Gene ID: | 22858 |
| NCBI Accession: | NP_055735.1 |
| UniProt Secondary Accession: | Q9UPZ9,O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, A7MD41 |
| UniProt Related Accession: | Q9UPZ9 |
| Molecular Weight: | 71 kDa |
| NCBI Full Name: | serine/threonine-protein kinase ICK |
| NCBI Synonym Full Names: | intestinal cell kinase |
| NCBI Official Symbol: | ICK |
| NCBI Official Synonym Symbols: | ECO; MRK; LCK2 |
| NCBI Protein Information: | serine/threonine-protein kinase ICK |
| UniProt Protein Name: | Serine/threonine-protein kinase ICK |
| UniProt Synonym Protein Names: | Intestinal cell kinase; hICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MRK |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | ICK |
 | Western blot analysis of extracts of various cell lines, using ICK antibody (CAB7783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using ICK antibody (CAB7783) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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