Anti-HTR2C Antibody (RACO0362)
- SKU:
- RACO0362
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | HTR2C Antibody |
| Product SKU: | RACO0362 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human 5HT2C Receptor |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis. |
| シノニム: | 5-hydroxytryptamine receptor 2C (5-HT-2C) (5-HT2C) (5-HTR2C) (5-hydroxytryptamine receptor 1C) (5-HT-1C) (5-HT1C) (Serotonin receptor 2C), HTR2C, HTR1C |
 | IHC image of RACO0362 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate A549 whole cell lysate All lanes HTR2C antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 52, 29 kDa. Observed band size: 75 kDa. |
| UniProt Protein Function: | 5-HT(2C): a G-protein coupled receptor. One of several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Mediates its action by activating a phosphatidylinositol-calcium second messenger system. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: Xq23 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:5-HT2 receptor activity; drug binding; neurotransmitter receptor activity; serotonin binding; serotonin receptor activity Biological Process: behavioral fear response; cellular calcium ion homeostasis; cGMP biosynthetic process; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of phosphatidylinositol biosynthetic process; regulation of appetite; regulation of corticotropin-releasing hormone secretion; regulation of neurological process; release of sequestered calcium ion into cytosol; response to drug; serotonin receptor signaling pathway; serotonin receptor, phospholipase C activating pathway; synaptic transmission |
| NCBI Summary: | This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants. [provided by RefSeq, Jan 2015] |
| UniProt Code: | P28335 |
| NCBI GenInfo Identifier: | 112816 |
| NCBI Gene ID: | 3358 |
| NCBI Accession: | P28335.1 |
| UniProt Secondary Accession: | P28335,Q5VUF8, Q9NP28, B1AMW4, |
| UniProt Related Accession: | P28335 |
| Molecular Weight: | 52kDa |
| NCBI Full Name: | 5-hydroxytryptamine receptor 2C |
| NCBI Synonym Full Names: | 5-hydroxytryptamine receptor 2C |
| NCBI Official Symbol: | HTR2C |
| NCBI Official Synonym Symbols: | HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C |
| NCBI Protein Information: | 5-hydroxytryptamine receptor 2C |
| UniProt Protein Name: | 5-hydroxytryptamine receptor 2C |
| UniProt Synonym Protein Names: | 5-hydroxytryptamine receptor 1C; 5-HT-1C; 5-HT1C; Serotonin receptor 2C |
| Protein Family: | 5-hydroxytryptamine receptor |
| UniProt Gene Name: | HTR2C |