Anti-HNMT Antibody (CAB2520)
- SKU:
- CAB2520
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-HNMT Antibody |
| 抗体コード: | CAB2520 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1). |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 22RV1, NCI-H460, HT-29, HepG2, HeLa, Mouse liver, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MASS MRSL FSDH GKYV ESFR RFLN HSTE HQCM QEFM DKKL PGII GRIG DTKS EIKI LSIG GGAG EIDL QILS KVQA QYPG VCIN NEVV EPSA EQIA KYKE LVAK TSNL ENVK FAWH KETS SEYQ SRML EKKE LQKW DFIH MIQM LYYV KDIP ATLK FFHS LLGT NAKM LIIV VSGS SGWD KLWK KYGS RFPQ DDLC QYIT SDDL TQML DNLG LKYE CYDL LSTM DISD CFID GNEN GDLL WDFL TETC NFNA TAPP DLRA ELGK DLQE PEFS AKKE GKVL FNNT LSFI VIEA |
| 遺伝子ID: | 3176 |
| Uniprot: | P50135 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 6kDa/14kDa/33kDa |
| 観察された分子量: | 33kDa |
| 同義語: | HNMT, HMT, HNMT-S1, HNMT-S2, MRT51 |
| バックグラウンド: | In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. |
| UniProt Protein Function: | HNMT: Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. Belongs to the methyltransferase superfamily. HNMT family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - histidine; EC 2.1.1.8; Methyltransferase Chromosomal Location of Human Ortholog: 2q22.1 Cellular Component: cytoplasm; cytosol; neuron projection; nucleoplasm Molecular Function:histamine N-methyltransferase activity Biological Process: brain development; histidine catabolic process; methylation; respiratory gaseous exchange Disease: Asthma, Susceptibility To |
| NCBI Summary: | In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50135 |
| NCBI GenInfo Identifier: | 1708272 |
| NCBI Gene ID: | 3176 |
| NCBI Accession: | P50135.1 |
| UniProt Secondary Accession: | P50135,Q546Z6, Q7Z7I2, Q8IU56, Q8WW98, Q9BRW6, B2R9J3 |
| UniProt Related Accession: | P50135 |
| Molecular Weight: | 6,046 Da |
| NCBI Full Name: | Histamine N-methyltransferase |
| NCBI Synonym Full Names: | histamine N-methyltransferase |
| NCBI Official Symbol: | HNMT |
| NCBI Official Synonym Symbols: | HMT; MRT51; HNMT-S1; HNMT-S2 |
| NCBI Protein Information: | histamine N-methyltransferase |
| UniProt Protein Name: | Histamine N-methyltransferase |
| Protein Family: | Histamine N-methyltransferase |
| UniProt Gene Name: | HNMT |
| UniProt Entry Name: | HNMT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HNMT antibody (CAB2520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using HNMT antibody (CAB2520). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug HNMT antibody (CAB2520). Western blot was performed from the immunoprecipitate using HNMT antibody (CAB2520) at a dilition of 1:1000.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s. |
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