Anti-HEY1 Antibody (CAB16110)
- SKU:
- CAB16110
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-HEY1 Antibody |
| 抗体コード: | CAB16110 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HEY1 (NP_001269780.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, 293T, Mouse heart, Mouse brain |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HEY1 (NP_001269780.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | IIEG LDAS DPLR VRLV SHLN NYAS QREA ASGA HAGL GHIP WGTV FGHH PHIA HPLL LPQN GHGN AGTT ASPT EPHH QGRL GSAH PEAP ALRA PPSG SLGP V |
| 遺伝子ID: | 23462 |
| Uniprot: | Q9Y5J3 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 32kDa/33kDa |
| 観察された分子量: | 35kDa |
| 同義語: | HEY1, BHLHb31, CHF2, HERP2, HESR1, HRT-1, OAF1, hHRT1 |
| バックグラウンド: | This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | HEY1: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'- CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Belongs to the HEY family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 8q21 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding; protein dimerization activity; transcription factor activity; transcription factor binding Biological Process: angiogenesis; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y5J3 |
| NCBI GenInfo Identifier: | 105990528 |
| NCBI Gene ID: | 23462 |
| NCBI Accession: | NP_036390.3 |
| UniProt Secondary Accession: | Q9Y5J3,Q5TZS3, Q8NAM2, Q9NYP4, B2R883, |
| UniProt Related Accession: | Q9Y5J3 |
| Molecular Weight: | 33 kDa |
| NCBI Full Name: | hairy/enhancer-of-split related with YRPW motif protein 1 isoform a |
| NCBI Synonym Full Names: | hes related family bHLH transcription factor with YRPW motif 1 |
| NCBI Official Symbol: | HEY1 |
| NCBI Official Synonym Symbols: | CHF2; OAF1; HERP2; HESR1; HRT-1; hHRT1; BHLHb31 |
| NCBI Protein Information: | hairy/enhancer-of-split related with YRPW motif protein 1 |
| UniProt Protein Name: | Hairy/enhancer-of-split related with YRPW motif protein 1 |
| UniProt Synonym Protein Names: | Cardiovascular helix-loop-helix factor 2; CHF-2; Class B basic helix-loop-helix protein 31; bHLHb31; HES-related repressor protein 1; Hairy and enhancer of split-related protein 1; HESR-1; Hairy-related transcription factor 1; HRT-1; hHRT1 |
| Protein Family: | Hairy/enhancer-of-split related with YRPW motif protein |
| UniProt Gene Name: | HEY1 |
| UniProt Entry Name: | HEY1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HEY1 antibody (CAB16110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of A431 cells using HEY1 antibody (CAB16110) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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