Anti-HDAC6 Antibody (RACO0137)
- SKU:
- RACO0137
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | HDAC6 Antibody |
| Product SKU: | RACO0137 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IP |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human HDAC6 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. |
| シノニム: | Histone deacetylase 6, HD6, HDAC6, KIAA0901, JM21 |
 | IHC image of RACO0137 diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunoprecipitating HDAC6 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of RACO0137 in HepG2 whole cell lysate For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0137 (3µg) + HepG2 whole cell lysate (500µg). Lane 3: HepG2 whole cell lysate (20µg). |
 | Western Blot. Positive WB detected in Hela whole cell lysate MCF-7 whole cell lysate HepG2 whole cell lysate K562 whole cell lysate Jurkat whole cell lysate All lanes HDAC6 antibody at 1 (1µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 132, 115 KDa. Observed band size: 160 KDa. |
| UniProt Protein Function: | HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; EC 3.5.1.98; Nuclear receptor co-regulator; Deacetylase Chromosomal Location of Human Ortholog: Xp11.23 Cellular Component: microtubule; dendrite; histone deacetylase complex; leading edge; perikaryon; caveola; inclusion body; cytosol; nucleoplasm; dynein complex; microtubule associated complex; axon; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasm; nucleus Molecular Function:zinc ion binding; microtubule binding; histone deacetylase binding; beta-catenin binding; beta-tubulin binding; Hsp90 protein binding; misfolded protein binding; actin binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); tubulin deacetylase activity; ubiquitin protein ligase binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; polyubiquitin binding; tau protein binding; alpha-tubulin binding Biological Process: negative regulation of proteolysis; response to misfolded protein; protein polyubiquitination; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; positive regulation of signal transduction; regulation of fat cell differentiation; macroautophagy; negative regulation of microtubule depolymerization; response to toxin; organelle organization and biogenesis; misfolded or incompletely synthesized protein catabolic process; histone deacetylation; regulation of gene expression, epigenetic; response to organic substance; intracellular protein transport; protein complex disassembly; lysosome localization; protein amino acid deacetylation; negative regulation of oxidoreductase activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent; negative regulation of protein complex disassembly Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia |
| NCBI Summary: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UBN7 |
| NCBI GenInfo Identifier: | 205371758 |
| NCBI Gene ID: | 10013 |
| NCBI Accession: | Q9UBN7.2 |
| UniProt Secondary Accession: | Q9UBN7,O94975, Q6NT75, Q7L3E5, Q96CY0, |
| UniProt Related Accession: | Q9UBN7 |
| Molecular Weight: | 114,361 Da |
| NCBI Full Name: | Histone deacetylase 6 |
| NCBI Synonym Full Names: | histone deacetylase 6 |
| NCBI Official Symbol: | HDAC6 |
| NCBI Official Synonym Symbols: | HD6; JM21; CPBHM; PPP1R90 |
| NCBI Protein Information: | histone deacetylase 6; protein phosphatase 1, regulatory subunit 90 |
| UniProt Protein Name: | Histone deacetylase 6 |
| UniProt Gene Name: | HDAC6 |
| UniProt Entry Name: | HDAC6_HUMAN |