Anti-HAGH Antibody (CAB6615)
- SKU:
- CAB6615
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-HAGH Antibody |
| 抗体コード: | CAB6615 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HAGH (NP_001035517.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HAGH (NP_001035517.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MKVE VLPA LTDN YMYL VIDD ETKE AAIV DPVQ PQKV VDAA RKHG VKLT TVLT THHH WDHA GGNE KLVK LESG LKVY GGDD RIGA LTHK ITHL STLQ VGSL NVKC LATP CHTS GHIC YFVS KPGG SEPP AVFT GDTL FVAG CGKF YEGT ADEM CKAL LEVL GRLP PDTR VYCG HEYT INNL KFAR HVEP GNAA IREK LAWA KEKY SIGE PTVP STLA EEFT YNPF MRVR EKTV QQHA GETD PVTT MRAV RREK DQFK MPRD |
| 遺伝子ID: | 3029 |
| Uniprot: | Q16775 |
| セルラーロケーション: | Cytoplasm, Mitochondrion matrix |
| 計算された分子量: | 25kDa/28kDa/33kDa |
| 観察された分子量: | 34kDa |
| 同義語: | HAGH, GLO2, GLX2, GLXII, HAGH1 |
| バックグラウンド: | The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | HAGH: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 3.1.2.6; Carbohydrate Metabolism - pyruvate; Hydrolase Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrial matrix; cytoplasm Molecular Function:hydroxyacylglutathione hydrolase activity; zinc ion binding Biological Process: glutathione biosynthetic process Disease: Hydroxyacyl Glutathione Hydrolase Deficiency |
| NCBI Summary: | The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] |
| UniProt Code: | Q16775 |
| NCBI GenInfo Identifier: | 257051015 |
| NCBI Gene ID: | 3029 |
| NCBI Accession: | Q16775.2 |
| UniProt Secondary Accession: | Q16775,A8K290, B4DP33, B4DRA7, E7EN93, |
| UniProt Related Accession: | Q16775 |
| Molecular Weight: | 308 |
| NCBI Full Name: | Hydroxyacylglutathione hydrolase, mitochondrial |
| NCBI Synonym Full Names: | hydroxyacylglutathione hydrolase |
| NCBI Official Symbol: | HAGH |
| NCBI Official Synonym Symbols: | GLO2; GLX2; GLXII; HAGH1 |
| NCBI Protein Information: | hydroxyacylglutathione hydrolase, mitochondrial; glx II; glyoxalase II; hydroxyacylglutathione hydroxylase |
| UniProt Protein Name: | Hydroxyacylglutathione hydrolase, mitochondrial |
| UniProt Synonym Protein Names: | Glyoxalase II; Glx II |
| UniProt Gene Name: | HAGH |
| UniProt Entry Name: | GLO2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HAGH antibody (CAB6615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using HAGH antibody (CAB6615) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using HAGH antibody (CAB6615) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using HAGH antibody (CAB6615). Blue: DAPI for nuclear staining. |
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