Anti-GTF3C4 Antibody (CAB9287)
- SKU:
- CAB9287
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-GTF3C4 Antibody |
| 抗体コード: | CAB9287 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | Jurkat, U-251MG, BT-474, HeLa, SW620 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ILYQ SMQK TPSE ALWK PTHE DSKI LLVD SPGM GNAD DEQQ EEGT SSKQ VVKQ GLQE RSKE GDVE EPTD DSLP TTGD AGGR EPME EKLL EIQG KIEA VEMH LTRE HMKR VLGE VYLH TWIT ENTS IPTR GLCN FLMS DEEY DDRT ARVL IGHI SKKM NKQT FPEH CSLC KEIL PFTD RKQA VCSN GHIW LRCF LTYQ SCQS LIYR RCLL HDSI ARHP APED PDWI KRLL QSPC PFCD SPVF |
| 遺伝子ID: | 9329 |
| Uniprot: | Q9UKN8 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 91kDa |
| 観察された分子量: | 95kDa |
| 同義語: | GTF3C4, KAT12, TF3C-delta, TFIII90, TFIIIC290, TFIIIC90, TFIIICDELTA |
| バックグラウンド: |
| UniProt Protein Function: | GTF3C4: Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box. Belongs to the TFIIIC subunit 4 family. |
| UniProt Protein Details: | Protein type:EC 2.3.1.48; Acetyltransferase Chromosomal Location of Human Ortholog: 9q34.13 Cellular Component: nucleoplasm; transcription factor TFIIIC complex Molecular Function:DNA binding; enzyme activator activity; protein binding Biological Process: 5S class rRNA transcription; transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; transcription, DNA-dependent; tRNA transcription from RNA polymerase III promoter |
| UniProt Code: | Q9UKN8 |
| NCBI GenInfo Identifier: | 212276467 |
| NCBI Gene ID: | 9329 |
| NCBI Accession: | Q9UKN8.2 |
| UniProt Secondary Accession: | Q9UKN8,Q5VZJ7, |
| UniProt Related Accession: | Q9UKN8 |
| Molecular Weight: | 91,982 Da |
| NCBI Full Name: | General transcription factor 3C polypeptide 4 |
| NCBI Synonym Full Names: | general transcription factor IIIC subunit 4 |
| NCBI Official Symbol: | GTF3C4 |
| NCBI Official Synonym Symbols: | KAT12; TFIII90; TFIIIC90; TFIIIC290; TF3C-delta; TFIIICDELTA |
| NCBI Protein Information: | general transcription factor 3C polypeptide 4 |
| UniProt Protein Name: | General transcription factor 3C polypeptide 4 |
| UniProt Synonym Protein Names: | TF3C-delta; Transcription factor IIIC 90 kDa subunit; TFIIIC 90 kDa subunit; TFIIIC90; Transcription factor IIIC subunit delta |
| Protein Family: | General transcription factor 3C polypeptide |
| UniProt Gene Name: | GTF3C4 |
| UniProt Entry Name: | TF3C4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (CAB9287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using Anti-GTF3C4 Polyclonal Antibody (CAB9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using Anti-GTF3C4 Polyclonal Antibody (CAB9287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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