Anti-GSTO1 Antibody (RACO0155)
- SKU:
- RACO0155
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | GSTO1 Antibody |
| Product SKU: | RACO0155 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human GSTO1 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. |
| シノニム: | Glutathione S-transferase omega-1, Glutathione S-transferase omega 1-1, GSTO 1-1, Glutathione-dependent dehydroascorbate reductase, GSTO1, GSTTLP28 |
 | IHC image of RACO0155 diluted at 1:125 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of RACO0155 diluted at 1:125 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in Jurkat whole cell lysate PC3 whole cell lysate Raji whole cell lysate K562 whole cell lysate Hela whole cell lysate A549 whole cell lysate HepG2 whole cell lysate All lanes GSTO1 antibody at 1.25µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 28, 24, 25 KDa. Observed band size: 28 KDa. |
| UniProt Protein Function: | GSTO1: Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. Belongs to the GST superfamily. Omega family. |
| UniProt Protein Details: | Protein type:EC 1.8.5.1; Transferase; EC 1.20.4.2; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450 Chromosomal Location of Human Ortholog: 10q25.1 Cellular Component: cytoplasm; cytosol Molecular Function:glutathione dehydrogenase (ascorbate) activity; glutathione transferase activity; methylarsonate reductase activity; oxidoreductase activity; protein binding Biological Process: glutathione metabolic process; L-ascorbic acid metabolic process; methylation; positive regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic catabolic process |
| NCBI Summary: | The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P78417 |
| NCBI GenInfo Identifier: | 6016173 |
| NCBI Gene ID: | 9446 |
| NCBI Accession: | P78417.2 |
| UniProt Secondary Accession: | P78417,Q5TA03, Q7Z3T2, D3DRA3, F5H7H0, |
| UniProt Related Accession: | P78417 |
| Molecular Weight: | 24,796 Da |
| NCBI Full Name: | Glutathione S-transferase omega-1 |
| NCBI Synonym Full Names: | glutathione S-transferase omega 1 |
| NCBI Official Symbol: | GSTO1 |
| NCBI Official Synonym Symbols: | P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21 |
| NCBI Protein Information: | glutathione S-transferase omega-1 |
| UniProt Protein Name: | Glutathione S-transferase omega-1 |
| UniProt Synonym Protein Names: | Glutathione S-transferase omega 1-1; GSTO 1-1; Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTO1 |
| UniProt Entry Name: | GSTO1_HUMAN |