Anti-GRK4 Antibody (CAB10370)
- SKU:
- CAB10370
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GRK4 Antibody |
| 抗体コード: | CAB10370 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 359-578 of human GRK4 (NP_892027.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, BT-474, HeLa, Mouse brain, Mouse lung, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 359-578 of human GRK4 (NP_892027.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | NNEK YTFS PDWW GLGC LIYE MIQG HSPF KKYK EKVK WEEV DQRI KNDT EEYS EKFS EDAK SICR MLLT KNPS KRLG CRGE GAAG VKQH PVFK DINF RRLE ANML EPPF CPDP HAVY CKDV LDIE QFSV VKGI YLDT ADED FYAR FATG CVSI PWQN EMIE SGCF KDIN KSES EEAL PLDL DKNI HTPV SRPN RGFF YRLF RRGG CLTM VPSE KEVE PKQC |
| 遺伝子ID: | 2868 |
| Uniprot: | P32298 |
| セルラーロケーション: | Cytoplasm, cell cortex |
| 計算された分子量: | 57kDa/61kDa/63kDa/66kDa |
| 観察された分子量: | 72kDa |
| 同義語: | GRK4, GPRK2L, GPRK4, GRK4a, IT11 |
| バックグラウンド: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | GRK4: Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:AGC group; EC 2.7.11.16; GRK family; GRK subfamily; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 4p16.3 Molecular Function:rhodopsin kinase activity Biological Process: receptor internalization; regulation of G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling |
| NCBI Summary: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| UniProt Code: | P32298 |
| NCBI GenInfo Identifier: | 143811400 |
| NCBI Gene ID: | 2868 |
| NCBI Accession: | P32298.3 |
| UniProt Secondary Accession: | P32298,O00641, O00642, Q13293, Q13294, Q13295, Q14453 Q14725, Q15313, Q15314, Q15315, Q15316, |
| UniProt Related Accession: | P32298 |
| Molecular Weight: | 67kDa |
| NCBI Full Name: | G protein-coupled receptor kinase 4 |
| NCBI Synonym Full Names: | G protein-coupled receptor kinase 4 |
| NCBI Official Symbol: | GRK4 |
| NCBI Official Synonym Symbols: | IT11; GPRK4; GRK4a; GPRK2L |
| NCBI Protein Information: | G protein-coupled receptor kinase 4 |
| UniProt Protein Name: | G protein-coupled receptor kinase 4 |
| UniProt Synonym Protein Names: | G protein-coupled receptor kinase GRK4; ITI1 |
| Protein Family: | G protein-coupled receptor kinase |
| UniProt Gene Name: | GRK4 |
 | Western blot analysis of extracts of various cell lines, using GRK4 antibody (CAB10370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
View AllClose