Anti-GRB2 Antibody (RACO0415)
- SKU:
- RACO0415
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | GRB2 Antibody |
| Product SKU: | RACO0415 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human, Mouse, Rat |
| 免疫原: | A synthesized peptide derived from human GRB2 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. |
| シノニム: | Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2), GRB2, ASH |
 | IHC image of RACO0415 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Hela whole cell lysate MCF-7 whole cell lysate Mouse Brain whole cell lysate Rat Brain whole cell lysate All lanes GRB2 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 26, 21 kDa. Observed band size: 28 kDa. |
| UniProt Protein Function: | Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. |
| NCBI Summary: | The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62993 |
| NCBI GenInfo Identifier: | 51702266 |
| NCBI Gene ID: | 2885 |
| NCBI Accession: | P62993.1 |
| UniProt Secondary Accession: | P62993,P29354, Q14450, Q63057, Q63059, |
| UniProt Related Accession: | P62993 |
| Molecular Weight: | 20,557 Da |
| NCBI Full Name: | Growth factor receptor-bound protein 2 |
| NCBI Synonym Full Names: | growth factor receptor bound protein 2 |
| NCBI Official Symbol: | GRB2 |
| NCBI Official Synonym Symbols: | ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2 |
| NCBI Protein Information: | growth factor receptor-bound protein 2 |
| UniProt Protein Name: | Growth factor receptor-bound protein 2 |
| UniProt Synonym Protein Names: | Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2 |
| Protein Family: | Growth factor receptor-bound protein |
| UniProt Gene Name: | GRB2 |
| UniProt Entry Name: | GRB2_HUMAN |