Anti-GOLGA5 Antibody (CAB15768)
- SKU:
- CAB15768
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GOLGA5 Antibody |
| 抗体コード: | CAB15768 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, Jurkat, HeLa, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSWF VDLA GKAE DLLN RVDQ GAAT ALSR KDNA SNIY SKNT DYTE LHQQ NTDL IYQT GPKS TYIS SAAD NIRN QKAT ILAG TANV KVGS RTPV EASH PVEN ASVP RPSS HFVR RKKS EPDD ELLF DFLN SSQK EPTG RVEI RKEK GKTP VFQS SQTS SVSS VNPS VTTI KTIE ENSF GSQT HEAA SNSD SSHE GQEE SSKE NVSS NAAC PDHT PTPN DDGK SHEL SNLR LENQ LLRN EVQS LNQE MASL LQRS KETQ EELN KARA RVEK WNAD HSKS DRMT RGLR AQVD DLTE AVAA KDSQ |
| 遺伝子ID: | 9950 |
| Uniprot: | Q8TBA6 |
| セルラーロケーション: | Golgi apparatus membrane, Single-pass type IV membrane protein |
| 計算された分子量: | 78kDa/83kDa |
| 観察された分子量: | 100kDa, 115kDa |
| 同義語: | GOLGA5, GOLIM5, RFG5, ret-II, golgin A5 |
| バックグラウンド: | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. |
| UniProt Protein Function: | GOLGA5: a member of the golgin family of proteins, whose members localize to the Golgi. Involved in regulating the architecture of the Golgi apparatus. A coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues. Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q32.12 Cellular Component: cis-Golgi network; Golgi apparatus; Golgi cisterna; Golgi membrane; integral to membrane; membrane; transport vesicle Molecular Function:protein homodimerization activity; Rab GTPase binding Biological Process: Golgi organization and biogenesis; Golgi vesicle transport; retrograde transport, vesicle recycling within Golgi Disease: Thyroid Carcinoma, Papillary |
| NCBI Summary: | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q8TBA6 |
| NCBI GenInfo Identifier: | 296439337 |
| NCBI Gene ID: | 9950 |
| NCBI Accession: | Q8TBA6.3 |
| UniProt Secondary Accession: | Q8TBA6,O95287, Q03962, Q2TS49, Q9UQQ7, C9JRU1, |
| UniProt Related Accession: | Q8TBA6 |
| Molecular Weight: | 78,108 Da |
| NCBI Full Name: | Golgin subfamily A member 5 |
| NCBI Synonym Full Names: | golgin A5 |
| NCBI Official Symbol: | GOLGA5 |
| NCBI Official Synonym Symbols: | RFG5; GOLIM5; ret-II |
| NCBI Protein Information: | golgin subfamily A member 5 |
| UniProt Protein Name: | Golgin subfamily A member 5 |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 31 protein; Golgin-84; Protein Ret-II; RET-fused gene 5 protein |
| Protein Family: | Golgin subfamily |
| UniProt Gene Name: | GOLGA5 |
| UniProt Entry Name: | GOGA5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GOLGA5 antibody (CAB15768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using GOLGA5 antibody (CAB15768) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using GOLGA5 antibody (CAB15768) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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