Anti-GNAS Antibody (CAB2732)
- SKU:
- CAB2732
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| 抗体名: | Anti-GNAS Antibody |
| 抗体コード: | CAB2732 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human GNAS |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | NCI-H460, SW620, Mouse skeletal muscle, Mouse brain, Mouse heart |
| 免疫原: | Recombinant protein of human GNAS |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 2778 |
| Uniprot: | O95467/P63092/P84996/Q5JWF2 |
| セルラーロケーション: | Cell membrane By similarity; Lipid-anchor By similarity |
| 計算された分子量: | 28kDa/44kDa/45kDa/77kDa/109kDa/111kDa |
| 観察された分子量: | 43kDa |
| 同義語: | GNAS, AHO, C20orf45, GNAS1, GPSA, GSA, GSP, NESP, POH, SCG6, SgVI |
| バックグラウンド: | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. |
| UniProt Protein Function: | G-alpha(s) iso7: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. |
| UniProt Protein Details: | Protein type:Oncoprotein; Membrane protein, peripheral; G protein; G protein, heterotrimeric; Vesicle; G protein, heterotrimeric alpha G(s) Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: cytoplasm; cytosol; dendrite; extracellular region; heterotrimeric G-protein complex; intrinsic to membrane; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; ruffle; trans-Golgi network membrane; transport vesicle Molecular Function:beta-2 adrenergic receptor binding; corticotropin-releasing hormone receptor 1 binding; D1 dopamine receptor binding; G-protein beta/gamma-subunit binding; GTP binding; GTPase activity; insulin-like growth factor receptor binding; ionotropic glutamate receptor binding; metal ion binding; mu-type opioid receptor binding; protein binding; signal transducer activity Biological Process: adenylate cyclase activation; cartilage development; cognition; developmental growth; DNA methylation; dopamine receptor, adenylate cyclase activating pathway; embryonic cranial skeleton morphogenesis; embryonic hindlimb morphogenesis; endochondral ossification; energy reserve metabolic process; female pregnancy; G-protein signaling, adenylate cyclase activating pathway; intracellular transport; metabolic process; multicellular organism growth; negative regulation of multicellular organism growth; positive regulation of cAMP biosynthetic process; positive regulation of GTPase activity; positive regulation of osteoblast differentiation; positive regulation of osteoclast differentiation; post-embryonic body morphogenesis; protein secretion; regulation of insulin secretion; regulation of signal transduction; renal water homeostasis; response to drug; sensory perception of chemical stimulus; sensory perception of smell; tissue homeostasis Disease: Acth-independent Macronodular Adrenal Hyperplasia; Mccune-albright Syndrome; Osseous Heteroplasia, Progressive; Pituitary Adenoma, Acth-secreting; Pituitary Adenoma, Growth Hormone-secreting; Pseudohypoparathyroidism, Type Ia; Pseudohypoparathyroidism, Type Ib; Pseudopseudohypoparathyroidism |
| NCBI Summary: | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012] |
| UniProt Code: | O95467 |
| NCBI GenInfo Identifier: | 74706023 |
| NCBI Gene ID: | 2778 |
| NCBI Accession: | O95467.1 |
| UniProt Secondary Accession: | O95467,O95417, B2RB88, E1P5G2, |
| UniProt Related Accession: | P63092,O95467,P84996,Q5JWF2 |
| Molecular Weight: | 111,025 Da |
| NCBI Full Name: | Neuroendocrine secretory protein 55 |
| NCBI Synonym Full Names: | GNAS complex locus |
| NCBI Official Symbol: | GNAS |
| NCBI Official Synonym Symbols: | AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; C20orf45 |
| NCBI Protein Information: | protein ALEX; protein GNAS; protein SCG6 (secretogranin VI) |
| UniProt Protein Name: | Neuroendocrine secretory protein 55 |
| UniProt Gene Name: | GNAS |
| UniProt Entry Name: | GNAS3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GNAS antibody (CAB2732) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |