Anti-GLDC Antibody (CAB9933)
- SKU:
- CAB9933
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-GLDC Antibody |
| 抗体コード: | CAB9933 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-290 of human GLDC (NP_000161.2). |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, DU145 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-290 of human GLDC (NP_000161.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DSSS GGGD SAAA GASR LLER LLPR HDDF ARRH IGPG DKDQ REML QTLG LASI DELI EKTV PANI RLKR PLKM EDPV CENE ILAT LHAI SSKN QIWR SYIG MGYY NCSV PQTI LRNL LENS GWIT QYTP YQPE VSQG RLES LLNY QTMV CDIT GLDM ANAS LLDE GTAA AEAL QLCY RHNK RRKF LVDP RCHP QTIA VVQT RAKY TGVL TELK LPCE MDFS GKDV SGVL FQYP DTEG KVED FTEL VERA HQSG SLA |
| 遺伝子ID: | 2731 |
| Uniprot: | P23378 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 112kDa |
| 観察された分子量: | 113kDa |
| 同義語: | GLDC, GCE, GCSP, HYGN1 |
| バックグラウンド: | Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). |
| UniProt Protein Function: | GLDC: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. Defects in GLDC are a cause of non-ketotic hyperglycinemia (NKH); also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. Belongs to the GcvP family. |
| UniProt Protein Details: | Protein type:EC 1.4.4.2; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine Chromosomal Location of Human Ortholog: 9p22 Cellular Component: cytosol; glycine cleavage complex; mitochondrial matrix; mitochondrion; nucleus; plasma membrane Molecular Function:electron carrier activity; glycine binding; glycine dehydrogenase (decarboxylating) activity Biological Process: glycine catabolic process; glycine decarboxylation via glycine cleavage system; glyoxylate metabolic process Disease: Glycine Encephalopathy |
| NCBI Summary: | Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010] |
| UniProt Code: | P23378 |
| NCBI GenInfo Identifier: | 229462870 |
| NCBI Gene ID: | 2731 |
| NCBI Accession: | P23378.2 |
| UniProt Secondary Accession: | P23378,Q2M2F8, |
| UniProt Related Accession: | P23378 |
| Molecular Weight: | 112,730 Da |
| NCBI Full Name: | Glycine dehydrogenase (decarboxylating), mitochondrial |
| NCBI Synonym Full Names: | glycine decarboxylase |
| NCBI Official Symbol: | GLDC |
| NCBI Official Synonym Symbols: | GCE; GCSP; HYGN1 |
| NCBI Protein Information: | glycine dehydrogenase (decarboxylating), mitochondrial |
| UniProt Protein Name: | Glycine dehydrogenase (decarboxylating), mitochondrial |
| UniProt Synonym Protein Names: | Glycine cleavage system P protein; Glycine decarboxylase; Glycine dehydrogenase (aminomethyl-transferring) |
| UniProt Gene Name: | GLDC |
| UniProt Entry Name: | GCSP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GLDC antibody (CAB9933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunoprecipitation analysis of 200ug extracts of DU145 cells using 3ug GLDC antibody (CAB9933). Western blot was performed from the immunoprecipitate using GLDC antibody (CAB9933) at a dilition of 1:1000. |
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