Anti-FTO Antibody (RACO0140)
- SKU:
- RACO0140
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | FTO Antibody |
| Product SKU: | RACO0140 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human FTO |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746). |
| シノニム: | Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, FTO, KIAA1752 |
 | IHC image of RACO0140 diluted at 1:70 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of RACO0140 diluted at 1:70 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in 293T whole cell lysate SH-SY5Y whole cell lysate Raji whole cell lysate Colo320 whole cell lysate A549 whole cell lysate PC3 whole cell lysate All lanes FTO antibody at 0.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 59, 15, 7, 13 KDa. Observed band size: 59 KDa. |
| UniProt Protein Function: | FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.11.- Chromosomal Location of Human Ortholog: 16q12.2 Cellular Component: nuclear speck; nucleus Molecular Function:ferrous iron binding; DNA-N1-methyladenine dioxygenase activity Biological Process: DNA dealkylation; RNA repair; regulation of multicellular organism growth; thermoregulation Disease: Growth Retardation, Developmental Delay, Coarse Facies, And Early Death |
| NCBI Summary: | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011] |
| UniProt Code: | Q9C0B1 |
| NCBI GenInfo Identifier: | 148841515 |
| NCBI Gene ID: | 79068 |
| NCBI Accession: | Q9C0B1.3 |
| UniProt Secondary Accession: | Q9C0B1,Q0P676, Q7Z785, A2RUH1, B2RNS0, |
| UniProt Related Accession: | Q9C0B1 |
| Molecular Weight: | 12,218 Da |
| NCBI Full Name: | Alpha-ketoglutarate-dependent dioxygenase FTO |
| NCBI Synonym Full Names: | fat mass and obesity associated |
| NCBI Official Symbol: | FTO |
| NCBI Official Synonym Symbols: | ALKBH9 |
| NCBI Protein Information: | alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; AlkB homolog 9; fat mass and obesity-associated protein |
| UniProt Protein Name: | Alpha-ketoglutarate-dependent dioxygenase FTO |
| UniProt Synonym Protein Names: | Fat mass and obesity-associated protein |
| Protein Family: | Alpha-ketoglutarate-dependent dioxygenase |
| UniProt Gene Name: | FTO |
| UniProt Entry Name: | FTO_HUMAN |