Anti-FOXA1 抗体 (CAB15278)

抗体名:	Anti-FOXA1 Antibody
抗体コード:	CAB15278
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 263-472 of human FOXA1 (NP_004487.2).

申し込み:	WB IHC
推奨希釈:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	Rat testis

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 263-472 of human FOXA1 (NP_004487.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	QKRF KCEK QPGA GGGG GSGS GGSG AKGG PESR KDPS GASN PSAD SPLH RGVH GKTG QLEG APAP GPAA SPQT LDHS GATA TGGA SELK TPAS STAP PISS GPGA LASV PASH PAHG LAPH ESQL HLKG DPHY SFNH PFSI NNLM SSSE QQHK LDFK AYEQ ALQY SPYG STLP ASLP LGSA SVTT RSPI EPSA LEPA YYQG VYSR PVLN TS
遺伝子ID:	3169
Uniprot:	P55317
セルラーロケーション:	Nucleus
計算された分子量:	45kDa/49kDa
観察された分子量:	50KDa

同義語:	FOXA1, HNF3A, TCF3A
バックグラウンド:	This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

UniProt Protein Function:	FOXA1: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles. Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of p27Kip1, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis- acting regulatory regions of these genes. Involved in glucose homeostasis. Binds DNA as a monomer. Interacts with FOXA2. Interacts with NKX2-1. Interacts with HDAC7. Interacts with the histone H3-H4 heterodimer. Associates with nucleosomes containing histone H2A. Interacts with AR. Interacts with NR0B2. Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 14q21.1 Cellular Component: nucleolus; nucleus Molecular Function:DNA binding; transcription factor activity Biological Process: chromatin remodeling; positive regulation of estrogen receptor signaling pathway; positive regulation of mitotic cell cycle; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to estradiol stimulus
NCBI Summary:	This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]
UniProt Code:	P55317
NCBI GenInfo Identifier:	296434509
NCBI Gene ID:	3169
NCBI Accession:	P55317.2
UniProt Secondary Accession:	P55317,Q9H2A0, B2R9H6, B7ZAP5,
UniProt Related Accession:	P55317
Molecular Weight:	45,468 Da
NCBI Full Name:	Hepatocyte nuclear factor 3-alpha
NCBI Synonym Full Names:	forkhead box A1
NCBI Official Symbol:	FOXA1
NCBI Official Synonym Symbols:	HNF3A; TCF3A
NCBI Protein Information:	hepatocyte nuclear factor 3-alpha
UniProt Protein Name:	Hepatocyte nuclear factor 3-alpha
UniProt Synonym Protein Names:	Forkhead box protein A1; Transcription factor 3A; TCF-3A
Protein Family:	Forkhead box protein
UniProt Gene Name:	FOXA1
UniProt Entry Name:	FOXA1_HUMAN

	Western blot analysis of extracts of Rat testis, using FOXA1 antibody (CAB15278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat lung using FOXA1 Rabbit pAb (CAB15278) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using FOXA1 Rabbit pAb (CAB15278) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using FOXA1 Rabbit pAb (CAB15278) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using FOXA1 Rabbit pAb (CAB15278) at dilution of 1:100 (40x lens).

Antibodies

Anti-FOXA1 Antibody (CAB9793)

FOXA1 Antibody (PACO02021)

Acetyl-FOXA1 (K264/253/211) Antibody (PACO06058)

FOXA1 Antibody (PACO09334)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)