Anti-FHOD1 Antibody (CAB15438)
- SKU:
- CAB15438
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-FHOD1 Antibody |
| 抗体コード: | CAB15438 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1010-1120 of human FHOD1 (NP_037373.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:200 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1010-1120 of human FHOD1 (NP_037373.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TRGR MITE TEKF SGVA GEAP SNPS VPVA VSSG PGRG DADS HASM KSLL TSRP EDTT HNRR SRGM VQSS SPIM PTVG PSTA SPEE PPGS SLPS DTSD EIMD LLVQ SVTK SSP |
| 遺伝子ID: | 29109 |
| Uniprot: | Q9Y613 |
| セルラーロケーション: | Cell projection, Cytoplasm, bleb, cytoskeleton |
| 計算された分子量: | 126kDa |
| 観察された分子量: | 145kDa |
| 同義語: | FHOD1, FHOS |
| バックグラウンド: | This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | FHOD1: a cytoskeletal protein required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C- terminus. |
| UniProt Protein Details: | Protein type:Actin-binding Chromosomal Location of Human Ortholog: 16q22 Cellular Component: cytoplasm; membrane; nucleus; stress fiber Molecular Function:identical protein binding; protein binding Biological Process: positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| UniProt Code: | Q9Y613 |
| NCBI GenInfo Identifier: | 62512187 |
| NCBI Gene ID: | 29109 |
| NCBI Accession: | Q9Y613.3 |
| UniProt Secondary Accession: | Q9Y613,Q59F76, Q6Y1F2, Q76MS8, Q8N521, |
| UniProt Related Accession: | Q9Y613 |
| Molecular Weight: | 126,551 Da |
| NCBI Full Name: | FH1/FH2 domain-containing protein 1 |
| NCBI Synonym Full Names: | formin homology 2 domain containing 1 |
| NCBI Official Symbol: | FHOD1 |
| NCBI Official Synonym Symbols: | FHOS |
| NCBI Protein Information: | FH1/FH2 domain-containing protein 1 |
| UniProt Protein Name: | FH1/FH2 domain-containing protein 1 |
| UniProt Synonym Protein Names: | Formin homolog overexpressed in spleen 1; FHOS; Formin homology 2 domain-containing protein 1 |
| Protein Family: | FH1/FH2 domain-containing protein |
| UniProt Gene Name: | FHOD1 |
| UniProt Entry Name: | FHOD1_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using FHOD1 antibody (CAB15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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