Anti-FDFT1 Antibody (RACO0450)
- SKU:
- RACO0450
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | FDFT1 Antibody |
| Product SKU: | RACO0450 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human FDFT1 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | endoplasmic reticulum, endoplasmic reticulum membrane, integral component of membrane, farnesyl-diphosphate farnesyltransferase activity, squalene synthase activity, cholesterol biosynthetic process, farnesyl diphosphate metabolic process, regulation of cholesterol biosynthetic process, regulation of lipid metabolic process, steroid biosynthetic process |
| シノニム: | Squalene synthase (SQS) (SS) (EC 2.5.1.21) (FPP:FPP farnesyltransferase) (Farnesyl-diphosphate farnesyltransferase), FDFT1 |
 | IHC image of RACO0450 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | IHC image of RACO0450 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in THP-1 whole cell lysate K562 whole cell lysate All lanes FDFT1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 49, 41, 39, 36, 44 kDa. Observed band size: 50 kDa. |
| UniProt Protein Function: | FDFT1: a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 2.5.1.21; Endoplasmic reticulum; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; Transferase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 8p23.1-p22 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane Molecular Function:farnesyl-diphosphate farnesyltransferase activity Biological Process: cholesterol biosynthetic process; steroid biosynthetic process |
| NCBI Summary: | This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P37268 |
| NCBI GenInfo Identifier: | 585126 |
| NCBI Gene ID: | 2222 |
| NCBI Accession: | P37268.1 |
| UniProt Secondary Accession: | P37268,Q96GT0, B3KQ95, B4DJE5, B4DT56, B7Z1J3, |
| UniProt Related Accession: | P37268 |
| Molecular Weight: | 43,060 Da |
| NCBI Full Name: | Squalene synthase |
| NCBI Synonym Full Names: | farnesyl-diphosphate farnesyltransferase 1 |
| NCBI Official Symbol: | FDFT1 |
| NCBI Official Synonym Symbols: | SS; SQS; DGPT; ERG9 |
| NCBI Protein Information: | squalene synthase |
| UniProt Protein Name: | Squalene synthase |
| UniProt Synonym Protein Names: | FPP:FPP farnesyltransferase; Farnesyl-diphosphate farnesyltransferase |
| UniProt Gene Name: | FDFT1 |
| UniProt Entry Name: | FDFT_HUMAN |