Anti-EPS8 Antibody (CAB14730)
- SKU:
- CAB14730
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-EPS8 Antibody |
| 抗体コード: | CAB14730 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human EPS8 (NP_004438.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-251MG, HeLa, HepG2, Mouse lung, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human EPS8 (NP_004438.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MNGH ISNH PSSF GMYP SQMN GYGS SPTF SQTD REHG SKTS AKAL YEQR KNYA RDSV SSVS DISQ YRVE HLTT FVLD RKDA MITV DDGI RKLK LLDA KGKV WTQD MILQ VDDR AVSL IDLE SKNE LENF PLNT IQHC QAVM HSCS YDSV LALV CKEP TQNK PDLH LFQC DEVK ANLI SEDI ESAI SDSK GGKQ KRRP DALR |
| 遺伝子ID: | 2059 |
| Uniprot: | Q12929 |
| セルラーロケーション: | Cell junction, Cell projection, Cytoplasm, cell cortex, growth cone, ruffle membrane, stereocilium, synapse, synaptosome |
| 計算された分子量: | 62kDa/91kDa |
| 観察された分子量: | 100kDa |
| 同義語: | EPS8, DFNB102 |
| バックグラウンド: | This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
| UniProt Protein Function: | EPS8: an adaptor protein that is an epidermal growth factor receptor (EGFR) substrate. Enhances EGF-dependent mitogenic signals. Member of a family of actin barbing and capping proteins. Promotes filopodial protrusions. Complexes containing Eps8/Abi1/PI3K/Sos-1 mediate Rac activation following EGFR signaling. Involved in endocytosis of EGFR by binding to the Rab5-specific GTPase activating protein USP6N. Binds to IRSp53, which binds small the small GTPases Rac and Cdc42, and which regulates actin dynamics. Involved in the actin-remodeling activity in cultured cerebellar neurons. Regulates the actin-remodeling activities of NMDA and ethanol in neurons. Eps8 knockout mice show behavioral resistance to ethanol. Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 12p12.3 Cellular Component: cell cortex; stereocilium; vesicle Molecular Function:actin binding; protein binding; Rac GTPase binding; SH3/SH2 adaptor activity Biological Process: actin crosslink formation; actin filament bundle formation; barbed-end actin filament capping; cell proliferation; epidermal growth factor receptor signaling pathway; exit from mitosis; Rac protein signal transduction; regulation of actin filament length; regulation of cell shape; signal transduction Disease: Deafness, Autosomal Recessive 102 |
| NCBI Summary: | This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12929 |
| NCBI GenInfo Identifier: | 2833239 |
| NCBI Gene ID: | 2059 |
| NCBI Accession: | Q12929.1 |
| UniProt Secondary Accession: | Q12929,Q8N6J0, A6NMC3, A8K6W2, A8KA66, B4DX66, |
| UniProt Related Accession: | Q12929 |
| Molecular Weight: | 62,871 Da |
| NCBI Full Name: | Epidermal growth factor receptor kinase substrate 8 |
| NCBI Synonym Full Names: | epidermal growth factor receptor pathway substrate 8 |
| NCBI Official Symbol: | EPS8 |
| NCBI Official Synonym Symbols: | DFNB102 |
| NCBI Protein Information: | epidermal growth factor receptor kinase substrate 8 |
| UniProt Protein Name: | Epidermal growth factor receptor kinase substrate 8 |
| Protein Family: | Epidermal growth factor receptor kinase substrate |
| UniProt Gene Name: | EPS8 |
| UniProt Entry Name: | EPS8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EPS8 antibody (CAB14730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s. |
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