Anti-DUSP4 Antibody (CAB2726)

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SKU:
CAB2726
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
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Description

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抗体名:Anti-DUSP4 Antibody
抗体コード:CAB2726
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC
反応性:Human
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human DUSP4 (NP_001385.1).
申し込み:WB IHC
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:100
反応性:Human
ポジティブサンプル:COS-7, HeLa, A375
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human DUSP4 (NP_001385.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MVTM EELR EMDC SVLK RLMN RDEN GGGA GGSG SHGT LGLP SGGK CLLL DCRP FLAH SAGY ILGS VNVR CNTI VRRR AKGS VSLE QILP AEEE VRAR LRSG LYSA VIVY DERS PRAE SLRE DSTV SLVV QALR RNAE RTDI CLLK GGYE RFSS EYPE FCSK TKAL AAIP
遺伝子ID:1846
Uniprot:Q13115
セルラーロケーション:Nucleus
計算された分子量:32kDa/42kDa
観察された分子量:47kDa
同義語:DUSP4, HVH2, MKP-2, MKP2, TYP
バックグラウンド:The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported.
UniProt Protein Function:MKP-2: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Induced by hepatocyte growth factor (HGF) and vascular endothelial cell growth factor (VEGF) in human endothelial cells. Two alternatively spliced isoforms have been described. In addition, multiple polyadenylation sites have been reported. Contains 1 rhodanese domain.
UniProt Protein Details:

Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: nucleoplasm; nucleus

Molecular Function:MAP kinase tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/threonine phosphatase activity

Biological Process: endoderm formation; inactivation of MAPK activity; innate immune response; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; protein amino acid dephosphorylation; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

NCBI Summary:The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:Q13115
NCBI GenInfo Identifier:2499745
NCBI Gene ID:1846
NCBI Accession:Q13115.1
UniProt Secondary Accession:Q13115,Q13524, B2RBU5, D3DSU4, G5E930,
UniProt Related Accession:Q13115
Molecular Weight:32,993 Da
NCBI Full Name:Dual specificity protein phosphatase 4
NCBI Synonym Full Names:dual specificity phosphatase 4
NCBI Official Symbol:DUSP4  
NCBI Official Synonym Symbols:TYP; HVH2; MKP2; MKP-2  
NCBI Protein Information:dual specificity protein phosphatase 4
UniProt Protein Name:Dual specificity protein phosphatase 4
UniProt Synonym Protein Names:Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2; MAP kinase phosphatase 2; MKP-2
Protein Family:Dual specificity protein phosphatase
UniProt Gene Name:DUSP4  
UniProt Entry Name:DUS4_HUMAN
Anti-DUSP4 Antibody (CAB2726)Western blot analysis of extracts of various cell lines, using DUSP4 antibody (CAB2726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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