Anti-DRD5 Antibody (CAB1719)
- SKU:
- CAB1719
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| 抗体名: | Anti-DRD5 Antibody |
| 抗体コード: | CAB1719 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 323-477 of human DRD5 (NP_000789.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 323-477 of human DRD5 (NP_000789.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SGHP EGPP AGFP CVSE TTFD VFVW FGWA NSSL NPVI YAFN ADFQ KVFA QLLG CSHF CSRT PVET VNIS NELI SYNQ DIVF HKEI AAAY IHMM PNAV TPGN REVD NDEE EGPF DRMF QIYQ TSPD GDPV AESV WELD CEGE ISLD KITP FTPN GFH |
| 遺伝子ID: | 1816 |
| Uniprot: | P21918 |
| セルラーロケーション: | Cell membrane, Multi-pass membrane protein |
| 計算された分子量: | 52kDa |
| 観察された分子量: | 55kDa |
| 同義語: | DRD5, DBDR, DRD1B, DRD1L2 |
| バックグラウンド: | This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. |
| UniProt Protein Function: | DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR Chromosomal Location of Human Ortholog: 4p16.1 Cellular Component: brush border membrane; integral to plasma membrane; nonmotile primary cilium; plasma membrane Molecular Function:dopamine binding; dopamine D1 receptor-like receptor activity; dopamine receptor activity Biological Process: adenylate cyclase activation; associative learning; cellular calcium ion homeostasis; dopamine receptor, adenylate cyclase activating pathway; dopamine receptor, phospholipase C activating pathway; G-protein signaling, adenylate cyclase activating pathway; learning; negative regulation of blood pressure; negative regulation of NAD(P)H oxidase activity; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; positive regulation of adenylate cyclase activity; regulation of female receptivity; regulation of systemic arterial blood pressure by vasopressin; response to amphetamine; response to cocaine; sensitization; synaptic transmission; synaptic transmission, dopaminergic; transmission of nerve impulse; wound healing Disease: Attention Deficit-hyperactivity Disorder; Blepharospasm, Benign Essential |
| NCBI Summary: | This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P21918 |
| NCBI GenInfo Identifier: | 118214 |
| NCBI Gene ID: | 1816 |
| NCBI Accession: | P21918.2 |
| UniProt Secondary Accession: | P21918,Q8NEQ8, B2R9S3, |
| UniProt Related Accession: | P21918 |
| Molecular Weight: | 52,951 Da |
| NCBI Full Name: | D(1B) dopamine receptor |
| NCBI Synonym Full Names: | dopamine receptor D5 |
| NCBI Official Symbol: | DRD5 |
| NCBI Official Synonym Symbols: | DBDR; DRD1B; DRD1L2 |
| NCBI Protein Information: | D(1B) dopamine receptor |
| UniProt Protein Name: | D(1B) dopamine receptor |
| UniProt Synonym Protein Names: | D(5) dopamine receptor; D1beta dopamine receptor; Dopamine D5 receptor |
| Protein Family: | D(1B) dopamine receptor |
| UniProt Gene Name: | DRD5 |
| UniProt Entry Name: | DRD5_HUMAN |
 | Western blot analysis of extracts of Mouse brain, using DRD5 antibody (CAB1719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using DRD5 antibody (CAB1719). Blue: DAPI for nuclear staining. |