Anti-DNAJA3 Antibody (CAB7030)
- SKU:
- CAB7030
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| 抗体名: | Anti-DNAJA3 Antibody |
| 抗体コード: | CAB7030 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_005138.3). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, Jurkat, 293T, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_005138.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AKGV NKEF TVNI MDTC ERCN GKGN EPGT KVQH CHYC GGSG METI NTGP FVMR STCR RCGG RGSI IISP CVVC RGAG QAKQ KKRV MIPV PAGV EDGQ TVRM PVGK REIF ITFR VQKS PVFR RDGA DIHS DLFI SIAQ ALLG GTAR AQGL YETI NVTI PPGT QTDQ KIRM GGKG IPRI NSYG YGDH YIHI KIRV PKRL TSRQ QSLI LSYA EDET DVEG TVNG VTLT SSGG STMD SSAG SKAR REAG EDEE GFLS KLKK MFTS |
| 遺伝子ID: | 9093 |
| Uniprot: | Q96EY1 |
| セルラーロケーション: | Cell junction, Cytoplasm, Mitochondrion matrix, Peripheral membrane protein, cytosol, postsynaptic cell membrane, synapse |
| 計算された分子量: | 33kDa/49kDa/52kDa |
| 観察された分子量: | 52kDa |
| 同義語: | DNAJA3, HCA57, TID1, hTID-1 |
| バックグラウンド: | This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. |
| NCBI Summary: | This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q96EY1 |
| NCBI GenInfo Identifier: | 311033374 |
| NCBI Gene ID: | 9093 |
| NCBI Accession: | Q96EY1.2 |
| UniProt Related Accession: | Q96EY1 |
| Molecular Weight: | 33kDa; 49kDa; 52kDa |
| NCBI Full Name: | DnaJ homolog subfamily A member 3, mitochondrial |
| NCBI Synonym Full Names: | DnaJ heat shock protein family (Hsp40) member A3 |
| NCBI Official Symbol: | DNAJA3 |
| NCBI Official Synonym Symbols: | TID1; HCA57; hTID-1 |
| NCBI Protein Information: | dnaJ homolog subfamily A member 3, mitochondrial |
| UniProt Protein Name: | DnaJ homolog subfamily A member 3, mitochondrial |
| UniProt Synonym Protein Names: | DnaJ protein Tid-1; hTid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog |
| Protein Family: | DnaJ homolog subfamily |
| UniProt Gene Name: | DNAJA3 |
 | Western blot analysis of extracts of various cell lines, using DNAJA3 antibody (CAB7030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat brain using DNAJA3 Antibody (CAB7030) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using DNAJA3 Antibody (CAB7030) at dilution of 1:100 (40x lens). |
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